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Tc2 automated cell counter

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The TC2 automated cell counter is a laboratory instrument designed to count and analyze cells. It provides accurate and consistent cell counts, enabling researchers to make informed decisions in their experiments.

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Lab products found in correlation

Axiovert 40 cfl microscope, by Nikon (2 mentions) Coolpix 4500 camera, by Nikon (2 mentions) Cls 1, by Worthington (2 mentions) Trypan blue solution, by Corning (1 mentions)

4 protocols using tc2 automated cell counter

1

VEGF Quantification by ELISA

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VEGF was measured by enzyme-linked immunosorbent assay (ELISA) kit from R&D (Minneapolis, MN). Cells seeded onto 6-well plates at a density of 4 × 104 cells per well were incubated for 24 h in complete media, and subjected to treatments in triplicate. Subsequent media were collected for ELISA, while cells were harvested for cell number counting with a TC2 automated cell counter (Bio-Rad, Hercules, CA). Triplicate measurements were made for each sample.
Zhong H., Wang R., Kelavkar U., Wang C.Y, & Simons J. (2014). Enzyme 15-lipoxygenase 1 promotes hypoxia-inducible factor 1α turnover and reduces vascular endothelial growth factor expression: implications for angiogenesis. Cancer Medicine, 3(3), 514-525.
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2

Cell Growth Quantification Protocol

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Cells (5 × 104) were plated in triplicate in 12-well plates. Cells were prepared with Trypan blue solution (430166, Corning Inc.) at 1:1 ratio and then were counted after 24, 48, and 72 hours using a TC2 Automated Cell Counter (1450102, Bio-Rad).
Jeong M.H., Urquhart G., Lewis C., Chi Z, & Jewell J.L. (2023). Inhibition of phosphodiesterase 4D suppresses mTORC1 signaling and pancreatic cancer growth. JCI Insight, 8(13), e158098.
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3

3D Collagen Cell Culture and Analysis

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Established cell lines (5 × 103) were suspended in neutralized rat tail collagen type I (cat # 354236 Corning) at 2 mg/mL and 250 μL was dispensed in 48-well plates and maintained for 7 days (Ebine et al., 2019 (link)). The morphology of the cells growing in 3D collagen was analyzed from photographs taken using Zeiss Axiovert 40 CFL microscope and Nikon Coolpix 4500 camera. Cells were extracted out of collagen gels with collagenase (10 mg/mL, Worthington Biochem #CLS1) solution by incubating at 37°C for 20 min and single cells generated by incubating with trypsin/EDTA (0.05% Trypsin/0.53mM EDTA, Corning #25052CI) for 3–5 min. Cells were counted using trypan blue and the TC2 automated cell counter (BioRad).
Shields M.A., Spaulding C., Metropulos A.E., Khalafalla M.G., Pharm T.N, & Munshi H.G. (2022). Gα13 loss in Kras/Tp53 mouse model of pancreatic tumorigenesis promotes tumors susceptible to rapamycin. Cell reports, 38(9), 110441.
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4

3D Collagen Cell Culture and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Established cell lines (5 × 103) were suspended in neutralized rat tail collagen type I (cat # 354236 Corning) at 2 mg/mL and 250 μL was dispensed in 48-well plates and maintained for 7 days (Ebine et al., 2019 (link)). The morphology of the cells growing in 3D collagen was analyzed from photographs taken using Zeiss Axiovert 40 CFL microscope and Nikon Coolpix 4500 camera. Cells were extracted out of collagen gels with collagenase (10 mg/mL, Worthington Biochem #CLS1) solution by incubating at 37°C for 20 min and single cells generated by incubating with trypsin/EDTA (0.05% Trypsin/0.53mM EDTA, Corning #25052CI) for 3–5 min. Cells were counted using trypan blue and the TC2 automated cell counter (BioRad).
Shields M.A., Spaulding C., Metropulos A.E., Khalafalla M.G., Pharm T.N, & Munshi H.G. (2022). Gα13 loss in Kras/Tp53 mouse model of pancreatic tumorigenesis promotes tumors susceptible to rapamycin. Cell reports, 38(9), 110441.
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