Log In Sign up
The largest database of trusted experimental protocols

11r vivit

Manufactured by Bio-Techne
Visit Supplier

11R-VIVIT is a lab equipment product from Bio-Techne. It is a device designed for use in scientific research applications.

Automatically generated - may contain errors

Lab products found in correlation

Interleukin 2 (il 2), by Thermo Fisher Scientific (2 mentions) Interleukin 7 (il 7), by Thermo Fisher Scientific (2 mentions) Fk506, by Bio-Techne (2 mentions) Erk phosphorylation, by Cell Signaling Technology (2 mentions) Nmu 23, by Phoenix Pharmaceuticals (2 mentions) Pd98059, by Merck Group (2 mentions)

Close spelling variants of this product

VIVIT

2 protocols using 11r vivit

1

NmU23 Activation of ILC2s Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified ILC2s from small intestine and lung were FBS starved for 2 hours before in vitro activation with NmU23 at 37°C. To test for ERK phosphorylation (Cell Signaling Technology), purified ILC2s were activated with NmU23 (100ng/mL; Phoenix Pharmaceuticals) in the presence of IL-2 and IL-7 (10ng/mL; PeproTech) for 10 minutes prior to intracellular staining. To test ERK, calcineurin and NFAT activation, ILC2s were cultured for 1 hour with their respective inhibitor and then stimulated with NmU23 overnight before mRNA expression analysis. ERK inhibitor - PD98059 (40μM; Sigma); Calcineurin inhibitor - FK506 (100nM) and CsA (100nM) (Tocris Bioscience); NFAT inhibitor - 11R-VIVIT (10μM) (Tocris Bioscience).
Cardoso V., Chesné J., Ribeiro H., García-Cassani B., Carvalho T., Bouchery T., Shah K., Barbosa-Morais N.L., Harris N, & Veiga-Fernandes H. (2017). Neuronal regulation of type 2 innate lymphoid cells via neuromedin U. Nature, 549(7671), 277-281.
+ Open protocol
+ Expand
2

NmU23 Activation of ILC2s Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified ILC2s from small intestine and lung were FBS starved for 2 hours before in vitro activation with NmU23 at 37°C. To test for ERK phosphorylation (Cell Signaling Technology), purified ILC2s were activated with NmU23 (100ng/mL; Phoenix Pharmaceuticals) in the presence of IL-2 and IL-7 (10ng/mL; PeproTech) for 10 minutes prior to intracellular staining. To test ERK, calcineurin and NFAT activation, ILC2s were cultured for 1 hour with their respective inhibitor and then stimulated with NmU23 overnight before mRNA expression analysis. ERK inhibitor - PD98059 (40μM; Sigma); Calcineurin inhibitor - FK506 (100nM) and CsA (100nM) (Tocris Bioscience); NFAT inhibitor - 11R-VIVIT (10μM) (Tocris Bioscience).
Cardoso V., Chesné J., Ribeiro H., García-Cassani B., Carvalho T., Bouchery T., Shah K., Barbosa-Morais N.L., Harris N, & Veiga-Fernandes H. (2017). Neuronal regulation of type 2 innate lymphoid cells via neuromedin U. Nature, 549(7671), 277-281.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!