MPM imaging was performed as described48 (link). Briefly, images were acquired on an upright Leica SP5 MP microscope (Leica Microsystems) equipped with two Mai Tai Ti:Sapphire lasers (Spectra Physics) and 10-Watt pumps or on an inverted Leica TCS SP8 MP microscope (Leica Microsystems). Ears were immobilized on an imaging platform and bathed in warm saline. All images were acquired using a 20x objective (NA 1.00). Emitted fluorescence was collected with a four-channel non-descanned detector. For blue/green channels, wavelength separation was accomplished with a dichroic mirror at 495 nm followed by emission filters of 460/50 nm bandpass and 525/50 nm bandpass. Second harmonic generation of collagen in the dermis is shown in blue according to convention. Tile-scans of 8 fields of view were collected covering approximately 2.8 mm of the ear. Sequences of image stacks were transformed into maximum intensity projections using Imaris software (Bitplane).
Sp5 mp microscope
Manufactured by Leica
The Leica SP5 MP microscope is a high-performance confocal laser scanning microscope designed for advanced imaging applications. It features a multi-photon excitation capability, allowing for deep tissue imaging and reduced phototoxicity. The instrument's core function is to provide researchers with a versatile and powerful tool for capturing detailed, high-resolution images of a wide range of biological samples.
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Lab products found in correlation
2 protocols using sp5 mp microscope
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Multiphoton Microscopy Imaging of Ear Tissue
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Multiphoton Microscopy Imaging of Ear Tissue
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MPM imaging was performed as described48 (link). Briefly, images were acquired on an upright Leica SP5 MP microscope (Leica Microsystems) equipped with two Mai Tai Ti:Sapphire lasers (Spectra Physics) and 10-Watt pumps or on an inverted Leica TCS SP8 MP microscope (Leica Microsystems). Ears were immobilized on an imaging platform and bathed in warm saline. All images were acquired using a 20x objective (NA 1.00). Emitted fluorescence was collected with a four-channel non-descanned detector. For blue/green channels, wavelength separation was accomplished with a dichroic mirror at 495 nm followed by emission filters of 460/50 nm bandpass and 525/50 nm bandpass. Second harmonic generation of collagen in the dermis is shown in blue according to convention. Tile-scans of 8 fields of view were collected covering approximately 2.8 mm of the ear. Sequences of image stacks were transformed into maximum intensity projections using Imaris software (Bitplane).
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