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Slide angiogenesis ibitreat microscopy chamber

Manufactured by Ibidi
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Sourced in Germany

The µ-Slide Angiogenesis ibiTreat Microscopy Chamber is a specialized lab equipment designed for microscopy applications. It provides a controlled environment for observing and analyzing angiogenesis, the formation of new blood vessels, under a microscope.

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Lab products found in correlation

Lsm 700, by Zeiss (2 mentions) Ix71 inverted microscope, by Olympus (1 mentions) Matrigel matrix, by BD (1 mentions)

Close spelling variants of this product

Chamber slides (62 citations) µ-Slide Angiogenesis (49 citations) IbiTreat µ-slides (14 citations) IbiTreat chamber slides (10 citations) IbiTreat chambers (9 citations) IbiTreat microscopy chamber (8 citations) Microscopy chambers (8 citations) µ-slide angiogenesis chambers (5 citations) Ibitreat angiogenesis slides (4 citations) Angiogenesis ibiTreat (3 citations)

2 protocols using slide angiogenesis ibitreat microscopy chamber

1

Angiogenesis Assay with HUVECs

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As previously described, transfected or drug-treated HUVECs were plated in µ-Slide Angiogenesis ibiTreat Microscopy Chamber (Ibidi, Martinsried, Germany) precoated with BD Matrigel Matrix (BD Biosciences, Bedford, MA) for 24 h. Images were captured by Olympus IX71 Inverted Microscope (Olympus, Japan) and total tube lengths were analyzed by Image J Angiogenesis Analyzer (NIH).
Zhao X., Yi Y., Meng C, & Fang N. (2019). MiRNA-575 suppresses angiogenesis by targeting Rab5-MEK-ERK pathway in endothelial cells. Bioscience Reports, 39(1), BSR20181218.
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2

Live/Dead Assay for 3D Cell Culture

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Pre-gel solution (10µl) containing 500,000 cells/ml were loaded into µ-Slide Angiogenesis ibiTreat microscopy chamber (Ibidi, Proxylab, Beloeil, BE) and were allowed to gel (n=3, N=3). After 2 days, a Live/Dead assay was performed. Pictures (2 fields/sample, Z-stack: 1 picture every 3µm, for a total of 210 pictures/stack) were then acquired with a confocal microscope (LSM700, Zeiss, Zaventem, BE). All the pictures were merged to count dead and living cells. Cell viability was expressed as a percentage of the number of living cells compared to the total number of cells.
Viswanath A., Vanacker J., Germain L., Leprince J.G., Diogenes A., Shakesheff K.M., White L.J, & des Rieux A. (2017). Extracellular matrix-derived hydrogels for dental stem cell delivery. Journal of biomedical materials research. Part A, 105(1).
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