Tandem mass spectrometry

Manufactured by Waters Corporation

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Tandem mass spectrometry is an analytical technique that combines two or more mass spectrometers to provide high-sensitivity and high-resolution analysis of complex samples. The core function of this equipment is to separate, identify, and quantify different molecules within a sample based on their mass-to-charge ratio.

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Lab products found in correlation

Radioimmunoassay, by DiaSorin (1 mentions) Masslynx software, by Waters Corporation (1 mentions) Ultra performance liquid chromatography 1 class, by Waters Corporation (1 mentions)

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Liquid chromatography-tandem mass spectrometry (2 citations) Triple quadrupole tandem mass spectrometry instrumentation (2 citations) Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry (2 citations) Xevo TQ-S tandem-quadrupole mass spectrometry (2 citations)

3 protocols using tandem mass spectrometry

Maternal Serum 25(OH)D Measurement

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Non-fasted venous blood samples were obtained at 11 and 34 weeks gestation and an aliquot of maternal serum stored at −80°C. The early pregnancy 25(OH)D samples were all analysed in a single batch in 2013, and the late pregnancy samples similarly in a single batch in 2008.
From the early pregnancy samples, serum 25(OH)D was analysed using high-performance liquid chromatography and tandem mass spectrometry: serum samples had an internal standard added, followed by protein denaturation by the addition of zinc sulphate and methanol. The internal standard and both 25(OH)D₂ and 25(OH)D₃ were then extracted into hexane, which was dried, and reconstituted in mobile phase. The extracts were analysed by liquid chromatography with detection by tandem mass spectrometry (Waters, Milford, MA, USA). From the late pregnancy samples, serum 25(OH)D concentrations were analysed by radioimmunoassay (Diasorin, Minnesota, USA). This assay measures both 25(OH)D₂ and 25(OH)D₃. Total 25(OH)D was calculated from the sum of 25(OH)D₂ and 25(OH)D₃ for both early and late pregnancy. The laboratories that undertook both analyses are members of the Vitamin D EQA scheme (DEQAS) and both assays met the requirements for this. The intra- and inter-assay coefficients of variation for both methods were <10%.

Moon R.J., Crozier S.R., Dennison E.M., Davies J.H., Robinson S.M., Inskip H.M., Godfrey K.M., Cooper C, & Harvey N.C. (2015). Tracking of 25-hydroxyvitamin D status during pregnancy: the importance of vitamin D supplementation. The American journal of clinical nutrition, 102(5), 1081-1087.

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Untargeted Metabolite Profiling via UPLC-MS/MS

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The cecal contents (50 mg) were vortexed in methanol/water solution (1 : 1 v/v, 1 ml) and centrifuged at 12,000 × g and 4°C for 5 min to collect the supernatant. Then the supernatant (50 μl) was derivatized in 50 μl derivatizing reagent and analyzed with UPLC-MS/MS. UPLC-MS/MS analysis was performed using an Ultra Performance Liquid Chromatography I-Class (Waters Co., Ltd., USA) coupled with a Tandem Mass Spectrometry (Waters Co., Ltd., USA). A 13-min linear gradient flow rate of 0.3 ml/min was used to inject samples into a UPLC RP Column (1.6 μm, 2.1 mm × 100 mm). Eluents A (0.1% formic acid in water) and B (methanol : isopropyl alcohol is 4 : 1) were used for the system. As follows, the solvent gradient: 5% B, 0 min; 5–15% B, 2.0 min; 15–55% B, 9.0 min; 55–100% B, 11.0 min; 100–5% B, 11.1 min; 5% B, 13 min. The Ultra Performance Liquid Chromatography operated with 150°C source temperature, 2.0 kV capillary voltage, 30.0 V cone voltage, 450°C desolvation temperature, 150 l/h cone gas flow, 1000 l/h desolvation gas flow, 0.15 ml/min collision gas flow rate. The collision gas used high-purity argon, and the rest of the gas paths used high-purity nitrogen. The data were analyzed by MassLynx software (Waters Co., Ltd., USA).

Li Y., Tong T., Li P., Peng Y., Zhang M., Liu J., She Y., Li Z, & Li Y. (2023). Screening of Potential Probiotic Lactobacillaceae and Their Improvement of Type 2 Diabetes Mellitus by Promoting PI3K/AKT Signaling Pathway in db/db Mice. Polish Journal of Microbiology, 72(3), 285-297.

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Quantification of Rilpivirine Plasma Levels

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Rilpivirine plasma concentrations were retrospectively measured at VF using ultraperformance liquid chromatography combined with tandem mass spectrometry (Waters Corporation, Milford, MA) as previously described [21 (link)].

Cazanave C., Reigadas S., Mazubert C., Bellecave P., Hessamfar M., Le Marec F., Lazaro E., Peytavin G., Bruyand M., Fleury H., Dabis F, & Neau D. (2015). Switch to Rilpivirine/Emtricitabine/Tenofovir Single-Tablet Regimen of Human Immunodeficiency Virus-1 RNA-Suppressed Patients, Agence Nationale de Recherches sur le SIDA et les Hépatites Virales CO3 Aquitaine Cohort, 2012–2014. Open Forum Infectious Diseases, 2(1), ofv018.

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