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Anti junb

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

Anti-JunB is a lab equipment product produced by Santa Cruz Biotechnology. It is an antibody that specifically binds to the JunB protein, which is a transcription factor involved in various cellular processes. The primary function of Anti-JunB is to detect and quantify the presence of JunB in biological samples.

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Lab products found in correlation

Dynabeads protein g, by Thermo Fisher Scientific (2 mentions) Ab8580, by Abcam (2 mentions) Ab1791, by Abcam (2 mentions) Ab8898, by Abcam (2 mentions) Ab6002, by Abcam (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Anti actin, by Beyotime (1 mentions) Rpmi 1640 medium, by GenePharma (1 mentions) Anti tgfbr1, by Santa Cruz Biotechnology (1 mentions) Anti ha, by Merck Group (1 mentions) Typhoon rgb imager, by GE Healthcare (1 mentions) Pierce ecl plus, by Thermo Fisher Scientific (1 mentions) Imagequant tl 1d software, by GE Healthcare (1 mentions) Anti c jun, by Cell Signaling Technology (1 mentions) Anti cd45.1 a20, by BioLegend (1 mentions) Anti cd45.2 104, by BioLegend (1 mentions) Anti cd4 gk1, by BioLegend (1 mentions) Anti cd3 17a2, by BioLegend (1 mentions) Anti ifn γ xmg1, by BioLegend (1 mentions) Anti rorγt q31 378, by BD (1 mentions)

Close spelling variants of this product

Anti-JunB (210) (2 citations) Anti-JunB (sc-73) (2 citations)

5 protocols using anti junb

1

ChIP-seq protocol for histone modifications

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ChIP was performed as described (Gal-Yam et al., 2008 (link)). Approximately 100 μg of chromatin isolated from 107 Karpas-299 or SU-DHL-1 cells were incubated with 50 μL of Dynabeads proteinG (Life Technologies) for 1 hr at 4°C and then with 10 μg of anti-JUNB (Santa Cruz, sc-73X) or 1 μg of anti-histone H3 (Abcam, ab1791), anti-H3K4me3 (Abcam, ab8580), anti-H3K9me3 (Abcam, ab8898), anti-H3K27me3 (Abcam, ab6002), or control immunoglobulin G (IgG) (Santa Cruz, sc-2027) overnight. Bound chromatin was washed, eluted, and de-cross-linked, and DNA was isolated by phenol-chloroform extraction after protein digestion. Primer sequences are listed in the Supplemental Experimental Procedures.
Hassler M.R., Pulverer W., Lakshminarasimhan R., Redl E., Hacker J., Garland G.D., Merkel O., Schiefer A.I., Simonitsch-Klupp I., Kenner L., Weisenberger D.J., Weinhaeusel A., Turner S.D, & Egger G. (2016). Insights into the Pathogenesis of Anaplastic Large-Cell Lymphoma through Genome-wide DNA Methylation Profiling. Cell reports, 17(2), 596-608.
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2

Culturing Kidney Cell Lines for Experiments

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The human kidney carcinoma cell line, 786-O, and the human embryonic kidney cell line, 293T, were purchased from American Type Culture Collection (ATCC). Cells were cultured in RPMI-1640 medium (Genepharma, Shanghai, China) supplemented with 10% fetal bovine serum (Invitrogen) in 5% CO2 at 37°C. The primary antibodies anti-TGFBR1 and anti-JunB were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and primary antibody anti-Actin was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Secondary antibodies anti-rabbit IgG and anti-mouse IgG were purchased from Beyotime and Santa Cruz, respectively.
He H., Wang L., Zhou W., Zhang Z., Wang L., Xu S., Wang D., Dong J., Tang C., Tang H., Yi X, & Ge J. (2015). MicroRNA Expression Profiling in Clear Cell Renal Cell Carcinoma: Identification and Functional Validation of Key miRNAs. PLoS ONE, 10(5), e0125672.
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3

Whole cell extract and Western blotting

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Whole cell extracts and western blotting were performed as described (Lemasson and Nyborg, 2001 (link)). Antibodies used were as follows: anti-c-Jun (Cell Signaling Technology, 9165) and anti-HA (Sigma Aldrich, H3663); anti-Myc (05–724), anti-actin (MAB1501), anti-FLAG M2 (F3165) were purchased from Millipore-Sigma; anti-JunB (Santa Cruz Biotech, N-17, sc-46). All blots were developed using Pierce ECL Plus (ThermoFisher) and scanned with a Typhoon RGB imager (GE Healthcare). Quantification of c-Jun bands was performed using Image Quant TL 1D software (version 8.1; GE Healthcare), c-Jun band volumes were normalized to the corresponding actin band volumes. The western blots shown are representative data from experiments performed at least twice.
Polakowski N., Pearce M., Kuguyo O., Boateng G., Hoang K, & Lemasson I. (2020). The splice 1 variant of HTLV-1 bZIP factor stabilizes c-Jun. Virology, 549, 51-58.
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4

Comprehensive Flow Cytometry and ChIP Analysis

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For flow cytometry analysis, the following antibodies were used at a 1:100 dilution: anti-CD3 (17A2; Biolegend), anti-CD4 (GK1.5; Biolegend), anti-CD25 (PC61; Biolegend), anti-CD44 (IM7; Biolegend), anti-CD62L (MEL-14; Biolegend), anti-CD45.1 (A20; Biolegend), anti-CD45.2 (104; Biolegend), anti-FasL (MFL3; Biolegend), anti-IL-17A (TC11-18H10.1; Biolegend), anti-IFN-γ (XMG1.2; Biolegend), anti-JunB (C-11; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-GATA3 (16E10A23; Biolegend), anti-ROR-γt (Q31-378; BD), anti-T-bet (4B10; Biolegend), and anti-rabbit IgG (Poly4064; Biolegend). For ChIP analyses, anti-JunB (1 μg per ChIP, 210; Santa Cruz), anti-BATF (1 μg per ChIP, WW8; Santa Cruz), anti-IRF4 (1 μg per ChIP, M-17; Santa Cruz) and rabbit IgG (1 μg per ChIP, I5006; Sigma) were used.
Hsieh T., Sasaki D., Taira N., Chien H., Sarkar S., Seto Y., Miyagi M, & Ishikawa H. (2022). JunB Is Critical for Survival of T Helper Cells. Frontiers in Immunology, 13, 901030.
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5

ChIP-seq protocol for histone modifications

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ChIP was performed as described (Gal-Yam et al., 2008 (link)). Approximately 100 μg of chromatin isolated from 107 Karpas-299 or SU-DHL-1 cells were incubated with 50 μL of Dynabeads proteinG (Life Technologies) for 1 hr at 4°C and then with 10 μg of anti-JUNB (Santa Cruz, sc-73X) or 1 μg of anti-histone H3 (Abcam, ab1791), anti-H3K4me3 (Abcam, ab8580), anti-H3K9me3 (Abcam, ab8898), anti-H3K27me3 (Abcam, ab6002), or control immunoglobulin G (IgG) (Santa Cruz, sc-2027) overnight. Bound chromatin was washed, eluted, and de-cross-linked, and DNA was isolated by phenol-chloroform extraction after protein digestion. Primer sequences are listed in the Supplemental Experimental Procedures.
Hassler M.R., Pulverer W., Lakshminarasimhan R., Redl E., Hacker J., Garland G.D., Merkel O., Schiefer A.I., Simonitsch-Klupp I., Kenner L., Weisenberger D.J., Weinhaeusel A., Turner S.D, & Egger G. (2016). Insights into the Pathogenesis of Anaplastic Large-Cell Lymphoma through Genome-wide DNA Methylation Profiling. Cell reports, 17(2), 596-608.
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