Cica liquid alt

Laboratory assays were performed with two different laboratory test companies. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and GGT levels were measured by the Japan Society of Clinical Chemistry recommended method, using a JCA-BM8060 (JEOL, Ltd., Tokyo, Japan) for the 2012 survey, and an AU5400 (Beckman Coulter, Inc., Brea, CA, USA) for the 2013 survey. Pureauto S AST, Pureauto S ALT, and Pureauto S GGT (Sekisui Medical Co., Tokyo, Japan) for the 2012 survey and Cica Luquid AST, Cica Liquid ALT and Cica Luquid GGT (Kanto Chemical Co., Tokyo Japan) for the 2013 survey were used as reagents. Serum ferritin levels were measured by a chemiluminescence immunoassay kit (Chemilumi ACS-Ferritin II; Siemens Healthcare Diagnostics, Tokyo, Japan) on an ADVIA Centaur (Bayer, Tarrytown, NY, USA).

To evaluate adverse effects of high dose probucol we measured 13 biomarkers using blood samples. Erythrocyte-, leukocyte-, and platelet-counts in blood samples were measured by using an automatic blood cell counter (Celltac α MEK-6458, Nihon Kohden, Tokyo, Japan). Blood aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), blood urea nitrogen, and their corresponding levels in serum were determined using CicaLiquid AST, CicaLiquid ALT, CicaLiquid LDH J, CicaLiquid-N UN (Kanto Chemical Co., Inc., Tokyo, Japan), respectively. Serum creatinine levels were determined using Determiner L CRE (Kyowa Medex Co., Ltd., Tokyo, Japan). Total protein levels in serum were determined using Clinimate TP (Sekisui Medical Co., Ltd., Tokyo, Japan). Serum concentrations of sodium, potassium and chloride were measured using Bio Majesty JCA-BM8060 (JEOL, Tokyo, Japan). Plasma cortisol levels were measured using electrochemiluminescence immunoassay (ECLIA, Roche Diagnostics, Mannheim, Germany).
Urine samples were analyzed for leukocyte, urobilinogen, occult blood reaction, bilirubin, ketonic metabolite, glucose, protein and pH values by using dipstick urinalysis (Uropaper III, Eiken Chemical Co., Ltd., Tokyo).