Formalin-fixed, paraffin-embedded tissues were immunohistochemically stained as described previously [18 (link)]. Primary antibodies against the following proteins were used: p53 (1:200, ab1101, Abcam, Cambridge, UK), Ki-67 (1:600, ab15580, Abcam), P-gp (1:800, 13978, CST, MA, USA), MRP1 (1:200, 72202, CST), Bcl2 (1:400, 15071, CST), TS (1:100, ab58287, Abcam), GST-π (1:200, ab58287, Abcam), and Bcl-2 (1:400, 12286, CST). Immunohistochemical staining was semi-quantitatively scored by rating staining intensity of a protein of interest (I: negative, 0; weak, 1; moderate, 2; intense, 3) and the percentage of positively stained cells (P: 0%–5%, scored 0; 6%–35%, scored 1; 36%–70%, scored 2; and > 70%, scored 3) to obtain a final score (Q), which was defined as the product of I × P. Two senior pathologists evaluated the tissues independently in a blinded manner.
Ab58287
Manufactured by Abcam
Sourced in United States
Ab58287 is a recombinant monoclonal antibody that recognizes Ras. The antibody is produced in E. coli and purified using protein A affinity chromatography.
Automatically generated - may contain errors
2 protocols using ab58287
1
Immunohistochemical Evaluation of Protein Expression
2
Antibody Validation for Western Blotting
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