Anti ifn β

Manufactured by R&D Systems

Sourced in France

Anti-IFN-β is a laboratory reagent used to neutralize and detect interferon-beta (IFN-β) in various experimental settings. It functions as an antibody that binds to IFN-β, allowing for the quantification and analysis of this cytokine.

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Lab products found in correlation

Anti ifnar2, by PBL Assay Science (1 mentions) Cpg2006, by InvivoGen (1 mentions) Sendai virus, by Charles River Laboratories (1 mentions) Hsv 1, by American Type Culture Collection (1 mentions) Anti β actin antibody, by Santa Cruz Biotechnology (1 mentions) Phospho jnk, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Phospho irf3, by Cell Signaling Technology (1 mentions) Phospho p65, by Cell Signaling Technology (1 mentions) Poly da dt, by InvivoGen (1 mentions) Cytotoxicity detection kit, by Roche (1 mentions) Ifn α, by Thermo Fisher Scientific (1 mentions)

3 protocols using anti ifn β

CpG Oligodeoxynucleotide Signaling in IFN-β Response

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Synthetic phosphodiester oligodeoxynucleotides (CpG 2006 and GpC 2006) were synthesized by InvivoGen (Toulouse, France) and used at indicated concentration. IFN-β was used at 100 or 1000 UI/ml (Avonex, Biogen, Nanterre Cedex, France), anti-IL-29, anti-IFNβ (R&D) and anti-IFNAR2 (PBL, New Jersey, NJ, USA). Cells were stimulated overnight and the response was monitored by luciferase assay or enzyme-linked immunosorbent assay. For luciferase assay transient transfection of the reporter plasmid NF-kB, or ELAM luciferase was performed as previously described.^{41 (link)} Enzyme-linked immunosorbent assays were done in accordance to manufacturer's instructions (R&D system).

Parroche P., Roblot G., Le Calvez-Kelm F., Tout I., Marotel M., Malfroy M., Durand G., McKay J., Ainouze M., Carreira C., Allatif O., Traverse-Glehen A., Mendiola M., Pozo-Kreilinger J.J., Caux C., Tommasino M., Goutagny N, & Hasan U.A. (2016). TLR9 re-expression in cancer cells extends the S-phase and stabilizes p16INK4a protein expression. Oncogenesis, 5(7), e244-.

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Antiviral Immune Response Assay

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Poly (dA:dT) was purchased from InvivoGen, c-di-GMP from KeraFast, cytotoxicity detection kit from Roche, HSV-1 (KOS strain) from ATCC and propagated in Vero cells, Sendai virus (Cantell strain) from Charles River and VSV (Indiana strain) from ATCC and propagated in Vero cells. TNF and IL-6 ELISA kits were from BD Biosciences, mouse anti-IFN-β antibody was from Cosmo Bio, anti-IFN-β was from R&D system, anti-phospho-TBK1, TBK1, phospho-IRF3, IRF3, phospho-p65, p65, phospho-JNK, phospho-ERK, phospho-p38 were from Cell Signaling Technology, and anti-β-actin antibody was from Santa Cruz.

Zhang L., Mo J., Swanson K.V., Wen H., Petrucelli A., Gregory S.M., Zhang Z., Schneider M., Jiang Y., Fitzgerald K.A., Ouyang S., Liu Z.J., Damania B.A., Shu H.B., Duncan J.A, & Ting J.P. (2014). NLRC3, a member of the NLR family of proteins, is a negative regulator of innate immune signaling induced by the DNA sensor STING. Immunity, 40(3), 329-341.

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Modulation of HCV Replicon Antiviral Activity

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Huh7/HCV-replicon cells were incubated with 10 μg/ml TNF-α
inhibitor (Enbrel, Immunex Corporation, Seattle, WA), 2 μg/ml IFN-γ R1
antibody or isotype control, 0.5 μg/ml IL-18 binding protein (all from R&D
Systems), 10 μg/ml anti-IFN-α (PBL Interferon Source, Piscataway, NJ) and
10 μg/ml anti-IFN-β (R&D Systems) or 0.2 μg/mL of the
vaccinia virus–encoded B18 receptor protein (VV B18R, eBiosciences), which
competes with the IFN-α/β receptor for IFN binding, prior to addition of
PBMC. After 24h, antiviral activity and IFN-γ production were determined as
described above.

Serti E., Werner J.M., Chattergoon M., Cox A.L., Lohmann V, & Rehermann B. (2014). Monocytes Activate Natural Killer Cells via Inflammasome-induced Interleukin 18 in Response to Hepatitis C Virus Replication. Gastroenterology, 147(1), 209-220.e3.

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