Tear lysozyme concentrations were measured using a Human Lysozyme enzyme-linked immunosorbent assay (ELISA) Kit (abcam, ab108880), and lactoferrin concentrations using a Human Lactoferrin ELISA Kit (Immunology Consultants Laboratory, E-80LF). For the Tanzanian cohort, tears were initially diluted 1:1000,000 for lysozyme quantification, and 1:100,000 for lactoferrin quantification. Tears were assayed in duplicate according to manufacturers’ instructions (n = 152 samples for both lysozyme and lactoferrin). Any samples with an absorbance too high to estimate lysozyme concentration from the standard curve, or with an estimated lysozyme concentration > 10ng/ml diluted, were tested at a dilution of 1:10,000,000. For the Gambian cohort, tears were diluted 1:25,000 for lysozyme quantification and 1:2500 for lactoferrin quantification. Tears were assayed in singlicate according to manufacturers’ instructions (n = 454 samples for lactoferrin and n = 377 samples for lysozyme). Concentrations were estimated from the standard curve using the drc package in R. For the Tanzanian cohort duplicates were averaged.
Human lysozyme enzyme linked immunosorbent assay elisa kit
Manufactured by Abcam
The Human Lysozyme Enzyme-Linked Immunosorbent Assay (ELISA) Kit is a quantitative in vitro diagnostic test used to measure the concentration of lysozyme in human samples. The kit utilizes the ELISA technique, which employs antibodies specific to human lysozyme for detection and quantification.
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2 protocols using human lysozyme enzyme linked immunosorbent assay elisa kit
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Quantifying Tear Lysozyme and Lactoferrin
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Quantifying Tear Lysozyme and Lactoferrin
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Tear lysozyme concentrations were measured using a Human Lysozyme enzyme-linked immunosorbent assay (ELISA) Kit (abcam, ab108880), and lactoferrin concentrations using a Human Lactoferrin ELISA Kit (Immunology Consultants Laboratory, E-80LF). For the Tanzanian cohort, tears were initially diluted 1:1000,000 for lysozyme quantification, and 1:100,000 for lactoferrin quantification. Tears were assayed in duplicate according to manufacturers’ instructions (n = 152 samples for both lysozyme and lactoferrin). Any samples with an absorbance too high to estimate lysozyme concentration from the standard curve, or with an estimated lysozyme concentration > 10ng/ml diluted, were tested at a dilution of 1:10,000,000. For the Gambian cohort, tears were diluted 1:25,000 for lysozyme quantification and 1:2500 for lactoferrin quantification. Tears were assayed in singlicate according to manufacturers’ instructions (n = 454 samples for lactoferrin and n = 377 samples for lysozyme). Concentrations were estimated from the standard curve using the drc package in R. For the Tanzanian cohort duplicates were averaged.
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