Thiotepa

Manufactured by Merck Group

Thiotepa is a pharmaceutical compound used as a laboratory reagent. It functions as an alkylating agent, a class of compounds that can modify and disrupt the structure of DNA molecules. Thiotepa is utilized in various research and analytical applications that require an agent capable of alkylating nucleic acids.

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Lab products found in correlation

2 protocols using thiotepa

Comprehensive Chemotherapeutic Reagent Procurement

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The following reagents were purchased from Sigma: MG-132, chlorambucil, cyclophosphamide, carmustine, busulfan, dacarbazine, thiotepa, cisplatin, 5-fluorouracil, 6-mercaptopurine, gemcitabine, methotrexate, doxorubicin, epirubicin, actinomycin-D, mitomycin-C, topotecan, irinotecan, etoposide, mitoxantrone, paclitaxel, docetaxel, and vincristine. The following reagents were purchased from Calbiochem: carboplatin, pentostatin, and daunorubicin. The following reagents were purchased from Santa Cruz: ICRF-187, ICRF-193, and teniposide. The following reagents were purchased from MBL International Corporation: Z-VAD-FMK.

Hsu J.M., Xia W., Hsu Y.H., Chan L.C., Yu W.H., Cha J.H., Chen C.T., Liao H.W., Kuo C.W., Khoo K.H., Hsu J.L., Li C.W., Lim S.O., Chang S.S., Chen Y.C., Ren G.X, & Hung M.C. (2018). STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion. Nature Communications, 9, 1908.

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Quantifying Secreted Albumin and CYP450 Activity

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Secreted albumin in flow-through from the devices was quantified by an enzyme-linked immunosorbent assay (ELISA) kit (Bethyl Labs) according to the manufacturer’s instructions. For enzyme induction experiments, microtissues were pretreated with inducers or inhibitors for 72 hours. Stock solutions were prepared in dimethyl sulfoxide (DMSO) and diluted at 1:1000 for final concentrations of 50 μM omeprazole (Sigma), 25 μM rifampin (Sigma), 25 μM 8-methoxypsoralen (Sigma), 50 μM Quinidine (Sigma), 50 μM ThioTEPA (Sigma). Phenobarbital (Sigma) was dissolved at 40 mM in deionized water and diluted to a final concentration of 1 mM. Vehicle controls were pretreated for 72 hours with DMSO or water. CYP450 activity was assessed by HPLC quantification of metabolites or with luminogenic P450-Glo^™ CYP450 assay kits (Promega) according to vendor instructions for non-lytic assays using cultured cells. Processed medium samples were collected from each strainer of microtissues or from flow-through from microfluidic devices and luciferin metabolites were measured on a luminometer (1 s; Tecan).

Schepers A., Li C., Chhabra A., Seney B.T, & Bhatia S. (2016). Engineering a perfusable 3D human liver platform from iPS cells. Lab on a chip, 16(14), 2644-2653.

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