Immuno-staining and image analysis were performed as described in McKim et al. (2009) (link) and Zhang et al. (2012a) (link). In short, 2–4 days old female ovaries were dissected in Robb’s buffer, fixed with 4% formaldehyde, washed, overnight incubated with primary antibody, washed, incubated overnight with secondary antibody with the fluorophore, stained with DAPI for DNA labeling and mounted on slide with mounting medium. To enhance the GFP signal, ChromoTek anti-GFP Booster (Atto-488) antibody was added with secondary antibody. Antibodies used: anti-GFP Booster (ChromoTek) at 1:200, guinea pig anti-Rhi (our lab) at 1:1000, rabbit anti-Del (from Julius Brennecke) at 1:1000, rabbit anti-TRF2 (from James Kadonaga) at 1:500, rabbit anti-H3K9me3 (abcam) at 1:1000.
Anti gfp booster atto 488
Manufactured by Proteintech
The Anti-GFP Booster (Atto-488) is a laboratory product designed to enhance the detection of green fluorescent protein (GFP) in experimental samples. It is a concentrated solution that can be added to GFP-containing samples to improve the fluorescent signal emitted by the GFP.
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Lab products found in correlation
2 protocols using anti gfp booster atto 488
1
Immuno-staining and Imaging of Drosophila Ovaries
2
Immuno-staining and Imaging of Drosophila Ovaries
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Immuno-staining and image analysis were performed as described in McKim et al. (2009) (link) and Zhang et al. (2012a) (link). In short, 2–4 days old female ovaries were dissected in Robb’s buffer, fixed with 4% formaldehyde, washed, overnight incubated with primary antibody, washed, incubated overnight with secondary antibody with the fluorophore, stained with DAPI for DNA labeling and mounted on slide with mounting medium. To enhance the GFP signal, ChromoTek anti-GFP Booster (Atto-488) antibody was added with secondary antibody. Antibodies used: anti-GFP Booster (ChromoTek) at 1:200, guinea pig anti-Rhi (our lab) at 1:1000, rabbit anti-Del (from Julius Brennecke) at 1:1000, rabbit anti-TRF2 (from James Kadonaga) at 1:500, rabbit anti-H3K9me3 (abcam) at 1:1000.
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