The catalase assay and glutathione peroxidase assay kits, acquired from Sigma-Aldrich, were employed to assess the intracellular activities of catalase and glutathione peroxidase, respectively, according to the manufacturer's instructions. On the other hand, the intracellular activities of peroxiredoxins were evaluated following a published protocol [25 ]. To ensure the veracity of our enzyme activity assessments, we incorporated affirmative benchmarks to validate the efficacy of the employed methodology. The catalase assay and glutathione peroxidase assay kits, procured from Sigma-Aldrich, were equipped with pre-included reagents serving as positive controls. Moreover, for the evaluation of peroxiredoxin activity, we acquired purified and functionally active peroxiredoxin from MyBioSource.com . Conversely, the negative controls exclusively consisted of reagents devoid of purified enzymes or cellular extracts.
Catalase assay
Manufactured by Merck Group
Sourced in United States
The Catalase Assay is a laboratory equipment used to measure the enzymatic activity of the catalase enzyme. Catalase is an important antioxidant enzyme that helps to break down hydrogen peroxide into water and oxygen. The assay provides a quantitative measure of the catalase activity in a given sample.
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Lab products found in correlation
2 protocols using catalase assay
1
Enzyme Activity Assays for Oxidative Stress
2
Assessing Oxidative Stress Biomarkers in Ovary
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For studying the involvement of oxidative stress, antioxidant enzyme activity of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione reductase (GR), Reduced nicotinamide adenine dinucleotide phosphate (NADPH) and thio-barbituric acid reactive substances (TBARS) were analysed. The isolated ovary tissue (20 mg) was homogenised using a tissue homogeniser in 2 ml phosphate buffer of pH7.4 followed by centrifugation at 10,000 g for 25 min at 4 °C. The supernatant of the resultant was used for measuring all the oxidative stress markers. The CAT and GR were evaluated using Catalase assay and glutathione reductase kit respectively (Sigma Aldrich USA), POD and NADPH by peroxidase assay and NADPH assay kit respectively (Abcam USA), SOD by superoxide dismutase kit (ThermoFisher USA), TBRAS using a TBRAS assay kit (Cayman Chemical, USA). All the procedures were in accordance to supplied instructions.
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