5 mm z gradient cp tci h c n cryogenic probe

All NMR spectra were recorded at 25
°C on a Bruker AVANCE III HD 800 MHz spectrometer equipped with
a 5 mm Z-gradient CP-TCI (H/C/N) cryogenic probe at the NV-NMR-Centre/Norwegian
NMR Platform (NNP) at the Norwegian University of Science and Technology
(NTNU). ¹H chemical shifts were internally referenced to
the water signal at 25 °C, while ¹⁵N and ¹³C chemical shifts were indirectly referenced to the water signal
based on absolute frequency ratios (Zhang et al. 2003).^{100 (link)} The NMR data were processed using TopSpin version
4.0.7. The processed NMR spectra
were analyzed using CARA version 1.5.5. The previously published NMR
assignment of SmLPMO10A (Aachmann et al., 2012) with
BMRB entry number 17160 was used.

All NMR spectra were recorded at 20 °C on a Bruker Avance III HD 800 MHz spectrometer using a 5 mm Z-gradient CP-TCI (H/C/N) cryogenic probe at the NV-NMR-Center/Norwegian NMR Platform (NNP) at the Norwegian University of Science and Technology (NTNU). ¹H signals were internally referenced to the water signal, and ¹³C and ¹⁵N signals were indirectly referenced to the water signal based on absolute frequency ratios (Zhang et al. 2003 (link)).
Backbone and side-chain resonance assignment were accomplished using ¹H–¹⁵N HSQC, ¹H–¹³C HSQC, HNCO, HNcaCO, HNCA, HNCACB. HNcoCACB, HNcoHAHB, HNHA, HcCH-TOCSY, and ¹⁵ N-NOESY-HSQC with 80 ms mixing time. All spectra were processed using TopSpin version 3.6.1.
Spectra were analysed using CARA (Computer Aided Resonance Assignment) version 1.8.4.2 (Keller 2004 ). A dihedral angle analysis based on the measured backbone and side-chain chemical shifts was made using TALOS-N (Shen and Bax 2013 (link)).