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Rabbit and mouse anti occludin

Manufactured by Thermo Fisher Scientific

The Rabbit and Mouse Anti-Occludin are primary antibodies that recognize the occludin protein. Occludin is a tight junction-associated protein that plays a crucial role in the regulation of epithelial and endothelial barrier function. These antibodies can be used to detect and study the expression and localization of occludin in various research applications.

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2 protocols using rabbit and mouse anti occludin

1

Immunofluorescence Staining of Tight Junctions

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Cell culture reagents were obtained from Mediatech Inc, Manassas, VA. The following antibodies were used: rabbit anti-GOPC (Millipore, Billerica, MA), anti-E-Cadherin (Cell Signaling, Danvers, MA), mouse anti-EEA1 (BD Biosciences, San Jose, CA), mouse anti-TGN38 (ABR Affinity Bioreagents, Golden, CO). Rat anti-E-Cadherin, mouse anti-G58K, mouse anti-GM130 and mouse anti-β-actin were from Sigma (Saint Louis, MO). Rabbit and mouse anti-claudin-1, rabbit and mouse anti-claudin-2, rabbit and mouse anti-occludin, mouse anti-claudin-4, mouse anti-ZO-1 were from Invitrogen (Grand Island, NY). Mouse anti-JAM-A was a gift from Dr. Charles Parkos, Emory University. AlexaFluor secondary antibodies were also from Invitrogen. All other chemicals and reagents were from Sigma-Aldrich unless otherwise specified.
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2

Epithelial Cell Tight Junction Analysis

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Cell culture reagents were obtained from Mediatech (Manassas, VA). The following antibodies were used: rabbit anti-Rab14 (AVIVA, San Diego, CA), rabbit anti-Rab14 (Sigma-Aldrich, St. Louis, MO), rat anti–E-cadherin (DECMA; Sigma-Aldrich), mouse anti-gp135 (gift from K. Matlin, University of Cincinnati, Cincinnati, OH), and rabbit anti–claudin-1, rabbit and mouse anti–claudin-2, mouse anti–claudin-4, and rabbit and mouse anti-occludin (all from Invitrogen, Grand Island, NY). Alexa Fluor secondary antibodies were also from Invitrogen. All other chemicals and reagents were from Sigma-Aldrich unless otherwise specified.
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