Jhoc 9

The OVTOKO, OVISE, OVMANA, RMG-I, OVSAHO, OVKATE, and OV1063 lines were purchased from the Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). The JHOC-7, JHOC-9, HTOA, JHOS-2, JHOS-3, and JHOS-4 cell lines were purchased from the RIKEN Cell Bank (Ibaraki, Japan). The TOV-21, ES-2, and SKOV3 cell lines were from the American Type Culture Collection (Manassas, VA). OVISE, OVTOKO, TOV-21G and ES2 were cultured in RPMI1640 medium containing 10% fetal bovine serum (FBS). OVMANA was cultured in RPMI medium containing 20% FBS, JHOC-7 in DMEM/F12 medium containing 10% FBS, JHOC-9 and RMG-I in DMEM/F12 medium containing 20% FBS, and SKOV3 in DMEM containing 10% FBS. The OVSAHO, OVKATE, OV1063, HTOA, JHOS-2, JHOS-3, and JHOS-4 lines were cultured in DMEM medium containing 10% FBS. The histological subtype of the SKOV3 cells was not unambiguously defined even after extensive analysis, although it was confidently identified as clear cell adenocarcinoma [22] (link). The immortalized epithelial cell line from an ovarian endometrial cyst was a generous gift from Dr. Satoru Kyo [23] (link).

RG7112 was purchased from MedChem Express (Monmouth Junction, NJ, USA) and APEXBIO (Houston, TX, USA). OVISE, OVTOKO, and RMG-I cell lines were purchased from the Japanese Collection of Research Bioresources Cell Bank (Ibaraki, Osaka, Japan), while cell lines JHOC-7 and JHOC-9 were purchased from RIKEN Cell Bank (Ibaraki, Tsukuba, Japan). ES-2 and SKOV3 cell lines were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). All these 7 cell lines are classified histologically as clear cell carcinoma, were cultured as described previously [48 (link)]. An immortalized cell line derived from ovarian surface epithelium was a generous gift from Dr. Hidetaka Katabuchi [53 (link)], and COS-7, and 293T cell lines were purchased from ATCC. The cell lines were authenticated by short tandem repeats.