Camptothecin (Sigma-Aldrich) was dissolved in DMSO and a stock solution (10 mM) was prepared and stored at −20°C. Iododeoxyuridine (IdU) and chlorodeoxyuridine (CldU) (Sigma-Aldrich) were dissolved in sterile DMEM to obtain stock solutions of 2.5 mM and 200 mM, respectively, and stored at −20°C. Mirin (Calbiochem), an inhibitor of MRE11 exonuclease activity, was used at 50 μM; the B02 compound (Selleck), an inhibitor of RAD51 activity, was used at 27 mM. The CDK inhibitor roscovitine (Selleck) was used at 20 μM.
Iododeoxyuridine
Manufactured by Merck Group
Iododeoxyuridine is a synthetic nucleoside analog used as a molecular biology research tool. It can be incorporated into DNA in place of thymidine, and is commonly used to label and track proliferating cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using iododeoxyuridine
1
DNA Replication Inhibitor Assay
2
Cell Cycle Dynamics in TUBG1 Mutant U2OS Cells
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U2OS, TUBG1-sgRNA-U2OS-TUBG1, and TUBG1-sgRNA-U2OS-TUBG1A429-A432-A435 cells were seeded at a density of 1.5 × 105 cells into 6-well plates. The cells were treated with 6 μM aphidicolin (Biochemica, #10797) for 24 h, followed by pulse-labeling with 25 μM chloro deoxyuridine (Sigma-Aldrich, C6891) for 30 min and sequentially with 250 μM iodo-deoxyuridine (Sigma-Aldrich, I7125) for 30 min. Collected cells were diluted to 5 × 104 cells/mL and then spread and stained as previously described52 (link). Images were acquired with a Zeiss LSM 780 inverted confocal microscope and analyzed with the ImageJ software.
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