Hp ultra 1

Manufactured by Agilent Technologies

Sourced in United States

The HP Ultra 1 is a laboratory equipment that provides high-performance analysis capabilities. It is designed for precise and accurate measurements, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

5975c mass spectrometer, by Agilent Technologies (1 mentions) 7890a gc system, by Agilent Technologies (1 mentions) 6890n network gc, by Agilent Technologies (1 mentions) 6890n network gas chromatograph, by Agilent Technologies (1 mentions) Heptadecanoic acid c17, by Merck Group (1 mentions) 5975c series, by Agilent Technologies (1 mentions) Bacterial acid methyl ester mix, by Merck Group (1 mentions)

4 protocols using hp ultra 1

GC-MS Analysis of APE(B/D) Fractions

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APE_B/D and its fractions were analyzed in GC/MS after different derivatizations methods. Samples were either hydrolyzed with 2m HCl/MeOH (1 h, 85°C), followed by a peracetylation (10 min., 85°C) or with 2m NaOH, followed by a methylation with trimethylsilyldiazomethane (30 min., 22°C) and silylation with N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) (3 h, 65°C). Additionally, to discriminate artifacts originating from acidic or alkaline hydrolysis APE_B/D was directly methylated (omitting the hydrolysis step) with trimethylsilyldiazomethane (30 min., 22°C) and silylated with BSTFA (3 h, 65°C).
GC-MS measurements were performed on Agilent Technologies 7890A gas chromatograph equipped with a dimethylpolysiloxane column (Agilent, HP Ultra 1; 12 m × 0.2 mm × 0.33 μm film thickness) and 5975C series MSD detector with electron impact ionization (EI) mode under autotune condition at 70 eV. The temperature programme was 70°C for 1.5 min, then 60°C min⁻¹ to 110°C and 5°C min⁻¹ to 320°C for 10 min.

González Roldán N., Engel R., Düpow S., Jakob K., Koops F., Orinska Z., Vigor C., Oger C., Galano J.M., Durand T., Jappe U, & Duda K.A. (2019). Lipid Mediators From Timothy Grass Pollen Contribute to the Effector Phase of Allergy and Prime Dendritic Cells for Glycolipid Presentation. Frontiers in Immunology, 10, 974.

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Bile Acid Profiling by LC-MS

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Plasma bile acid species were quantified from 25 μl plasma samples using LC-MS, as previously described (25) . To 25 μl of plasma, we added a mixture of internal standards (stable isotope-labelled bile acids) in methanol. Samples were centrifuged at 15 800 g, and the supernatant was transferred and evaporated at 40°C (26, 27) . The GC system consisted of 6890N network GC (Agilent Technologies) and was equipped with a HP-ULTRA 1 (50 m length × 0•2 mm diameter, 0•11 μm film thickness, Agilent) column. The GC-MS system consisted of a 7890A GC system coupled to a 5975C mass spectrometer (Agilent) with the same HP-ULTRA 1 column and used an ammonia gas carrier. Oleate methyl ester ammonium ions were monitored at m/z 314-318, palmitate methyl ester ions were monitored at m/z 288-292 and 303-304 and stearate methyl ester ions were monitored at m/z 316-320. Ions were monitored under selected ion recording. The normalised mass isotopomer distributions measured by GC-MS (m 0 -m x ) were corrected for natural abundance of 13 C by multiple linear regression, described in more detail elsewhere (28, 29) .

Ronda O.A.H.O., van de Heijning B.J.M., Martini I.A., Koehorst M., Havinga R., Jurdzinski A., Bloks V.W., van Dijk T.H., van der Beek E.M., Kuipers F, & Verkade H.J. (2021). An early-life diet containing large phospholipid-coated lipid globules programmes later-life postabsorptive lipid trafficking in high-fat diet- but not in low-fat diet-fed mice. The British journal of nutrition, 125(9).

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Quantification of Fatty Acid Methyl Esters

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Fatty acid methyl esters (FAMEs) were quantified using gas chromatography^{14 (link),48 (link)}. Cryogenically crushed tissues were homogenized in Potter–Elvehjem tubes in ice-cold phosphate buffered saline (PBS) solution. A known quantity of homogenized tissue, plasma or food was transferred to glass tubes, and capped with silicone-ptfe septum screw caps. An internal standard (heptadecanoic acid, C17, Sigma, St. Louis, MO, USA) was added. Lipids were trans-methylated at 90 °C for 4 h in 6 M HCl:methanol (ratio 1:5), liquid–liquid extracted twice using hexane, transferred to a clean tube, dried at 45 °C under a stream of N₂, reconstituted in hexane and transferred to GC vials with inserts. Samples were analyzed by gas chromatography as previously described^{48 (link)}. The GC system consisted of 6890N network gas chromatograph (Agilent) and was equipped with a HP- ULTRA 1 (50 m length × 0.2 mm diameter, 0.11 µm film thickness) column.

Ronda O.A., van de Heijning B.J., Martini I., Gerding A., Wolters J.C., van der Veen Y.T., Koehorst M., Jurdzinski A., Havinga R., van der Beek E.M., Kuipers F, & Verkade H.J. (2020). Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice. Scientific Reports, 10, 16128.

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Fatty Acid Profiling of MGlcDAG

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The fatty acid composition of MGlcDAG of the wild type and the ÁFabN mutant was determined after methanolysis (2 M HCl/MeOH, 85 C, 2 h), acetylation (85 C, 10 min) and detection by GC/MS. GC/MS analyses of all samples were performed on an Agilent Technologies 7890A gas chromatograph equipped with a dimethylpolysiloxane column [HP Ultra 1, 12 m Â 0.2 mm Â 0.33 mm film thickness and 5975C series MSD detector with electron impact ionization (EI) mode under autotune condition at 70 eV (Agilent, Santa Clara, CA, USA)]. The temperature program was 70 C for 1.5 min, then 60 C min À1 to 110 C and 5 C min À1 to 320 C for 10 min. A reference probe (Bacterial Acid Methyl Ester Mix; Sigma Aldrich) with known lipid composition and elution profile was measured under the same conditions.

Diederich A.K., Duda K.A., Romero-Saavedra F., Engel R., Holst O, & Huebner J. (2016). Deletion of fabN in Enterococcus faecalis results in unsaturated fatty acid auxotrophy and decreased release of inflammatory cytokines. Innate immunity, 22(4).

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