Aedes albopictus-derived C6/36 cells were maintained in Leibovitz’s L-15 medium (PAN Biotech, Aidenbach, Germany) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% tryptose phosphate broth (TPB) at 28 °C without additional CO2. BHK-21 cells (ATCC, CCL-10) were cultured in Glasgow’s minimal essential medium (GMEM; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS and 2% TPB at 37 °C in a humidified incubator with 5% CO2. All growth media were supplemented with 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).
Leibovitz s l 15 medium
Manufactured by PAN Biotech
Sourced in Germany
Leibovitz's L-15 medium is a cell culture medium formulated for supporting the growth and maintenance of various cell types. It is a balanced salt solution that provides essential nutrients, minerals, and energy sources required for cell proliferation.
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Lab products found in correlation
3 protocols using leibovitz s l 15 medium
1
Culturing Aedes albopictus and BHK-21 Cells
2
Culturing Human Melanoma Cell Lines
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Human melanoma cell lines (1205Lu, 451Lu, 451Lu‐BR3, WM983B, WM983B‐BR, and WM3734) and HEK293T cells for lentiviral production were provided by Meenhard Herlyn (The Wistar Institute, Philadelphia, USA). Cells were cultured in TU 2% medium (80% MCDB153 basal medium (#P04‐80062, PAN Biotech), 20% Leibovitz's L‐15 medium (#P04‐27055, PAN Biotech) supplemented with 2 mM L‐Glutamin, 1.68 mM CaCl2 and 2% FCS) and were maintained at 37°C in 5% CO2. All cell lines tested negative for Mycoplasma using regularly the PCR Mycoplasma Test Kit I/C (PromoKine #PK‐CA91‐1048, PromoCell GmbH, Heidelberg, Germany). Chemicals were purchased from Sigma‐Aldrich (Munich, Germany), unless otherwise indicated. Small molecule inhibitors were purchased from Selleckchem (Absource, Munich, Germany).
3
Labeling of Human Cancer Cells and Tissue
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Human cancer cell line SK-BR-3 was grown in RPMI 1640 medium (Biochrom AG, Berlin, Germany) supplemented with 10% (v/v) FCS. MDA-MB-231 cells were grown in Leibovitz’s L15 medium (PAN Biotech, Aidenbach, Germany) supplemented with 10% (v/v) FCS. Cancer cells were infected with adenovirus carrying GFP (Ad-GFP, Vector Biolab, Philadelphia, PA, USA).
PCLS fixed by 3.7% formaldehyde were permeabilized by 0.2% Triton-X 100 in PBS for 10 min at room temperature. Tissue was counterstained with 1.7 μM TAMRA-SE (Life technologies, Carlsbad, CA, USA) for 2.5 h at 4°C, washed once with 100 mM glycine and twice with PBS before staining the nuclei using 0.5 μM TO-PRO-3 iodide (Life technologies) for 1 h at 4°C. After additional washing, PCLS were mounted on glass coverslips in Mowiol- solution containing DABCO.
PCLS fixed by 3.7% formaldehyde were permeabilized by 0.2% Triton-X 100 in PBS for 10 min at room temperature. Tissue was counterstained with 1.7 μM TAMRA-SE (Life technologies, Carlsbad, CA, USA) for 2.5 h at 4°C, washed once with 100 mM glycine and twice with PBS before staining the nuclei using 0.5 μM TO-PRO-3 iodide (Life technologies) for 1 h at 4°C. After additional washing, PCLS were mounted on glass coverslips in Mowiol- solution containing DABCO.
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