Hadscs

Manufactured by PromoCell

Sourced in Germany

HADSCs are primary human adipose-derived stem cells. They are isolated from human adipose tissue and exhibit the characteristic properties of mesenchymal stem cells, including the ability to differentiate into various cell types such as adipocytes, osteoblasts, and chondrocytes.

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Lab products found in correlation

Fetal bovine serum (fbs), by Biowest (3 mentions) Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions) Mscgm cd mesencymal stem cell growth medium bulletkit, by Lonza (1 mentions) Recombinant human mfg e8 protein, by R&D Systems (1 mentions) L glutamine, by Fujifilm (1 mentions) Sodium pyruvate, by Fujifilm (1 mentions) Phenol red, by Fujifilm (1 mentions) Mscgm cd mesenchymal stem cell bulletkit, by Lonza (1 mentions) Trypsin, by Lonza (1 mentions)

4 protocols using hadscs

Culturing of Human Adipose-Derived Stem Cells

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The MSCGM-CDTM Mesenchymal Stem Cell Growth Medium BulletKit™ was obtained from Lonza (Basel, Switzerland). hADSCs (from a 46-year-old Caucasian female; PromoCell, Heidelberg, Germany) were cultured. FBS was obtained from BioWest (Nuaille, France). DMEM was obtained from Wako Pure Chemical Industries (Osaka, Japan). Plastic dishes were obtained from TPP (Trasadingen, Switzerland). All other materials used were of the highest commercial grade.

Nakashima Y., Nahar S., Miyagi-Shiohira C., Kinjo T., Kobayashi N., Saitoh I., Watanabe M., Fujita J, & Noguchi H. (2018). A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Cells Cultured in DMEM 10% FBS and Chemically Defined Medium Using Human Adipose-Derived Mesenchymal Stem Cells. International Journal of Molecular Sciences, 19(7), 2042.

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Mesenchymal Stem Cell Culture Optimization

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The MSCGM-CD™ Mesencymal Stem Cell Growth Medium BulletKit™ was obtained from Lonza (Basel, Switzerland). hADSCs (46-year-old/female/Caucasian) were obtained from PromoCell (Heidelberg, Germany). Fetal bovine serum (FBS) was obtained from BioWest (Nuaille, France). d-MEM (high glucose) with l-Glutamine, Phenol Red and Sodium Pyruvate (DMEM) was obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Recombinant Human MFG-E8 Protein was obtained from R&D Systems, Inc. (Minneapolis, MN, USA). Lipopolysaccharide (LPS) from Escherichia coli O26:B6 was obtained from MilliporeSigma (St. Louis, MO, USA). Plastic dishes were obtained from TPP (Trasadingen, Switzerland). All other materials were of the highest commercial grade.

Nakashima Y., Miyagi-Shiohira C., Noguchi H, & Omasa T. (2018). The Healing Effect of Human Milk Fat Globule-EGF Factor 8 Protein (MFG-E8) in A Rat Model of Parkinson’s Disease. Brain Sciences, 8(9), 167.

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Culturing hADSCs with FBS and DMEM

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Fetal bovine serum (FBS) was obtained from BioWest (Nuaille, Maine-et-Loire, France). Dulbecco’s modified Eagle medium (DMEM) [D-MEM (High Glucose) with L-glutamine, phenol red and sodium pyruvate (catalogue number 043-30085)] was obtained from Wako Pure Chemical Industries (Osaka, Japan). hADSCs (46-year-old Caucasian female) were purchased from PromoCell (Heidelberg, Germany). The plastic dishes were obtained from TPP (Trasadingen, Switzerland).

Nahar S., Nakashima Y., Miyagi-Shiohira C., Kinjo T., Kobayashi N., Saitoh I., Watanabe M., Noguchi H, & Fujita J. (2018). A Comparison of Proteins Expressed between Human and Mouse Adipose-Derived Mesenchymal Stem Cells by a Proteome Analysis through Liquid Chromatography with Tandem Mass Spectrometry. International Journal of Molecular Sciences, 19(11), 3497.

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Culturing and Passaging hADSCs

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hADSCs (46-year-old Caucasian female) (PromoCell, Heidelberg, Germany) were cultured (37 °C, 5% CO₂) on a coated 100 mm culture plate (TPP 93100). Then, after reaching 80% confluence passage of cells was performed every 3–4 days. The cells were washed with Phosphate buffered saline (PBS) (calcium, magnesium-free), and hADSCs were dissociated using a dissociation solution. Subculturing was carried out by plating on uncoated 100 mm culture plate. An MSCGM-CD mesenchymal stem cell BulletKit (Lonza 00190632) or DMEM-10% FBS was used for the culture medium. Trypsin/ethylenediaminetetraacetic acid (EDTA) (Lonza CC-3232) was used for the dissociation solution.

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