3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt

N-Cetyltrimethylammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), sodium hydroxide (NaOH), 3-aminopropyltriethoxysilane (APTES), pyruvic acid (PA), N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-(dimethylamino) propyl) carbodiimide hydrochloride (EDC), (2-thienylmethyl)hydrazine hydrochloride, (5,6-dimethylthieno[2,3-d]pyrimidin-4-yl)hydrazine, trifluoroacetic acid (TFA), triethylamine (TEA), ethanol, 2-propanol from Carl Roth (Carl Roth GmbH & Co. KG, Karlsruhe, Germany), dichloro(p-cymene)ruthenium(II) dimer, phosphate buffered saline (PBS), toluene and crystal violet (CV) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Biowest (Riverside, CA, USA). For in vitro experiments nutrition medium RPMI 1640, FCS (fetal calb serum), penicillin/streptomycin and phosphate buffer saline (PBS) were obtained from Capricorn scientific GmbH (Ebsdorfergrund, Germany), glutamine and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) from GE healthcare (Chicago, IL, USA) and Biomol GmbH (Hamburg, Germany), respectively.

HepG2 (C3A) hepatocytes (catalogue number: HB-8065) and L6 myocytes (catalogue number: JCRB9081) were purchased from Cellonex, South Africa. Fetal calf serum (FCS), Roswell Park Memorial Institute (RPMI) 1640 Medium, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and Dulbecco's Modified Eagle Medium (DMEM) were procured from GE Healthcare Life Sciences (Logan, UT, USA). Dimethyl sulfoxide (DMSO) and phosphate-buffered saline (PBS) (with and without Ca2+ and Mg2+ and trypsin) were purchased from Lonza (Walkersville, MD, USA). Bovine serum albumin (BSA), and insulin were purchased from Sigma (St. Louis, MO, USA).