PNGase F was obtained from New England Biolabs (Ipswich, MA, USA). Borane-ammonia complex, sodium hydroxide beads, acetic acid, and iodomethane were acquired from Sigma-Aldrich (St. Louis, MO, USA). Dimethyl sulfoxide (DMSO) was bought from Mallinckrodt Chemicals (Phillipsburg, NJ, USA). HPLC grade acetonitrile, water, and isopropanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Isolute® C18 (EC) cartridges were purchased from Biotage (Charlotte, NC, USA), and microspin columns were purchased from Harvard Apparatus (Holliston, MA, USA).
Isolute c18 ec cartridges
Manufactured by Biotage
Sourced in United States
Isolute® C18 (EC) cartridges are solid-phase extraction (SPE) products designed for sample preparation. They are composed of a silica-based sorbent modified with octadecyl (C18) functional groups. These cartridges are intended for the extraction, isolation, and purification of analytes from complex matrices.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide beads, by Merck Group (2 mentions)
Iodomethane, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Microspin columns, by Harvard Apparatus (2 mentions)
Borane ammonia complex, by Merck Group (1 mentions)
Isopropanol, by Thermo Fisher Scientific (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Pngase f, by New England Biolabs (1 mentions)
Dimethyl sulfoxide (dmso), by Mallinckrodt (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Ammonium borane complex, by Merck Group (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
High performance liquid chromatography grade water, by Thermo Fisher Scientific (1 mentions)
Luteolin, by Carl Roth (1 mentions)
Apigenin, by Merck Group (1 mentions)
Hcooh, by Merck Group (1 mentions)
Pheophorbide a, by Merck Group (1 mentions)
Ch3oh, by Avantor (1 mentions)
Lichroprep rp 18, by Merck Group (1 mentions)
4 protocols using isolute c18 ec cartridges
1
PNGase F Glycan Modification Protocol
2
Glycoprotein Analysis in Cerebrospinal Fluid
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Iodomethane, sodium hydroxide beads, acetic acid, and ammonium borane complex were purchased from Sigma Aldrich (St. Louis, MO, USA). Isolute® C18 (EC) cartridges were purchased from Biotage (Charlotte, NC, USA) and 10k Amicon Ultra-0.5 mL Centrifugal Filters were purchased from Millipore Sigma (Burlington, MA, USA). Microspin columns were purchased from Harvard Apparatus (Hollison, MA, USA). N-glycosidase F enzyme (PNGase F) was acquired from New England Biolabs (Ipswich, MA, USA). Solvents, including high-performance liquid chromatography (HPLC)-grade water, acetonitrile, methanol, and dimethyl sulfoxide were purchased from Fisher Scientific (Pittsburgh, PA, USA). Pooled CSF was acquired from Golden West Biologicals, Inc. (Temecula, CA, USA).
3
HPLC-HRMS Analysis of Plant Compounds
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For HPLC-HRMS analyses, H2O was purified with a Barnstead EASYpure RF compact ultrapure water system, and CH3OH (VWR International, Rosny-sous-Bois-cedex, France) and HCOOH (Sigma Aldrich, Steinheim, Germany) were LC-MS grade. The other solvents were of analytical grade. Amentoflavone, luteolin and luteolin-7-glucoside were purchased from Carl Roth (Karlsruhe, Germany). Apigenin and pheophorbide A were purchased from Sigma Aldrich (Steinheim, Germany), and podocarpusflavone A, from Carbosynth (Berkshire, UK). Silica gel 60 for VLC (particle size 0.040–0.063 mm) and LiChroprep® RP-18 (0.040–0.063 mm) for RP-18 column chromatography were purchased from Merck (Darmstadt, Germany). For solid-phase extraction, 5 g or 10 g Isolute® C18 (EC) cartridges (Biotage, Uppsala, Sweden) were used.
4
Urinary Cortisol Quantification in Damaraland Mole-rats
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For Experiment 1 and the non-experimental samples used for treatment validation, urinary cortisol was quantified using radioimmunoassay kits (Coat a Count, Diagnostic Products Corporation, Los Angeles, CA) validated for Damaraland mole-rats (Clarke et al., 2001) (link). For Experiment 2, a solid-phase extraction was performed using Isolute C18(EC) cartridges (50 mg/1 cc, Biotage) and urinary cortisol quantification was performed with ultra-highpressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The specific gravity of all urine samples was determined using a digital handheld refractometer (Atago Ltd) to adjust raw cortisol concentrations following Miller et al. (2004) (link) and correct for differences in urine concentrations. Details of the methods followed for sample preparation, analytical procedures, quality control and calculation of intra-and inter-assay coefficient of variation are provided in the electronic supplementary material.
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