Cells were lysed in RIPA buffer supplemented with 1x Halt proteinase inhibitor (87786, Thermo Scientific) and phosphatase inhibitor cocktail (BP479, Boston Bioproducts, Milford, MA, USA). The primary antibodies used include anti-DLST (H00001743, Abnova, Taipei, Taiwan), anti-IDH1 (LS-C497935, Life span bioscience, Seattle, WA, USA), anti-IDH2 (LS-C373619, Life span bioscience), anti-LDHA (#2012, Cell Signaling Technology, Danvers, MA, USA), and anti-ACTIN (sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA). Secondary antibodies include anti-mouse (31430, Thermo Scientific) or anti-rabbit antibodies (65-6120, Thermo Scientific). Chemiluminescence Supersignal West Pico was from Thermo Scientific (Cat# 34080). Autoradiographs were imaged using a G: BOX Chemi XT4 (Syngene, Bengaluru, India).
Phosphatase inhibitor cocktail
Manufactured by Boston BioProducts
Sourced in United States
Phosphatase inhibitor cocktail is a solution designed to inhibit the activity of phosphatases, which are enzymes responsible for the dephosphorylation of proteins. This product can be used to preserve the phosphorylation state of proteins during sample preparation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Supersignal west pico chemiluminescence, by Thermo Fisher Scientific (2 mentions)
Anti dlst, by Abnova (2 mentions)
Anti actin, by Santa Cruz Biotechnology (2 mentions)
G box chemi xt4, by Syngene (2 mentions)
Anti rabbit antibodies, by Thermo Fisher Scientific (1 mentions)
Anti ldha, by Cell Signaling Technology (1 mentions)
2 protocols using phosphatase inhibitor cocktail
1
Protein Expression Analysis in Cell Lysates
2
Western Blot Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed in RIPA buffer supplemented with 1× Halt proteinase inhibitor (87786, Thermo Scientific) and phosphatase inhibitor cocktail (BP479, Boston Bioproducts). Primary antibodies include anti-DLST (H00001743, Abnova) and anti-ACTIN (sc-47778, Santa Cruz Biotechnology). Secondary antibodies included goat anti-mouse (31430, Thermo Scientific) or anti-rabbit HRP antibodies (65-6120, Thermo Scientific). Chemiluminescence Supersignal West Pico was from Thermo Scientific (Cat# 34080). Autoradiographs were imaged using a G: BOX Chemi XT4 (Syngene).
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