Sybr green taqman pcr kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The SyBr green Taqman PCR kit is a molecular biology tool used for quantitative polymerase chain reaction (qPCR) analysis. It contains reagents and materials necessary to perform real-time PCR experiments, including fluorescent DNA-binding dyes and optimized buffers.

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Lab products found in correlation

Bicinchoninic acid protein assay kit, by Thermo Fisher Scientific (4 mentions) Rneasy kit, by Qiagen (4 mentions) Trizol reagent, by Thermo Fisher Scientific (3 mentions) Corticosterone elisa kit, by Cayman Chemical (2 mentions) Progesterone and testosterone elisa kits, by ALPCO (2 mentions) Superscript 2, by Thermo Fisher Scientific (2 mentions) Donkey antigoat igg irdye 680, by LI COR (2 mentions) Odyssey blocking buffer, by LI COR (2 mentions) Irdye 680 goat anti rabbit igg, by LI COR (2 mentions) Irdye 800 goat anti mouse igg, by LI COR (2 mentions) Cholesterol liquicolor test, by EKF Diagnostics (1 mentions) Qbt fatty acid uptake assay kit, by Molecular Devices (1 mentions) Insulin, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) 3h palmitic acid, by PerkinElmer (1 mentions) Bt2camp, by Merck Group (1 mentions) Recombinant mouse chemerin protein, by R&D Systems (1 mentions) Mab2325, by R&D Systems (1 mentions) Protein assay dye reagent concentrate, by Bio-Rad (1 mentions)

Close spelling variants of this product

TaqMan SYBR Green Taqman SYBR kit TaqMan PCR kit SYBR Green kit SYBR Green TaqMan kits

4 protocols using sybr green taqman pcr kit

Steroid Hormone Assay Protocol

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Reagents were obtained from the following sources: Cholesterol LiquiColor Test (enzymatic) from Stanbio (Boerne, TX); bicinchoninic acid assay protein kit from Pierce Biotechnology, Inc (Rockford, IL); organic solvents were from J. T. Baker (Phillipsburg, NJ); TRIzol reagent and SuperScript II were from Invitrogen (Carlsbad, CA); RNeasy kit from QIAGEN (Valencia, CA); SYBR Green TaqMan PCR kit from Applied Biosystems (Foster City, CA); corticosterone ELISA kit from Cayman Chemical (Ann Arbor, MI); progesterone and testosterone ELISA kits from ALPCO (Salem, NH); pregnant mare's serum gonadotropin (PMSG)/equine chorionic gonadotropin (eCG), human chorionic gonadotropin (hCG), and Bt₂cAMP from MilliporeSigma (St. Louis, MO). Cortrosyn was from Amphastar Pharmaceuticals, Inc (Rancho Cucamonga, CA).

Shen W.J., Cortez Y., Singh A., Chen W., Azhar S, & Kraemer F.B. (2022). Mice deficient in ER protein seipin have reduced adrenal cholesteryl ester lipid droplet formation and utilization. Journal of Lipid Research, 63(12), 100309.

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Cholesterol Uptake Measurement Protocol

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Reagents were obtained from the following sources: Cholesteryl BODIPY FLC12 (cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacence-3-dodecanoate) was obtained from Molecular Probes (Life Technologies, Grand Island, NY). Bicinchoninic acid assay protein kit was from Pierce Biotechnology, Inc. (Rockford, IL); organic solvents were from J. T. Baker (Phillipsburg, NJ); TRIzol reagent and SuperScript II were from Invitrogen (Carlsbad, CA); RNeasy kit was from QIAGEN (Valencia, CA); SyBr green Taqman PCR kit was from Applied Biosystems (Foster City, CA); Odyssey blocking buffer, goat antimouse IgG-IRDye 800, donkey antigoat IgG-IRDye 680, and goat antirabbit IgG-IRDye 680 were from Li-Cor Biosciences (Lincoln, NE); block lipid transport-1 (BLT-1), sulfo-N-succinimidyl oleate (SSO), isobutyl-methylxanthine, dexamethasone, and insulin were from Sigma-Aldrich (St. Louis, MO). QBT Fatty Acid Uptake Assay Kit was from Molecular Devices Corporation, Sunnyvale, CA, USA; siRNAs for mouse SR-B1 and CD36 were purchased from Thermo Fisher Scientific (Waltham, MA).

Wang W., Yan Z., Hu J., Shen W.J., Azhar S, & Kraemer F.B. (2019). Scavenger Receptor Class B, Type 1 Facilitates Cellular Fatty Acid Uptake. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865(2), 158554.

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Detailed Biochemical Assay Reagents

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Reagents were obtained from the following sources: Cholesterol LiquiColor Test (Enzymatic) from Stanbio (Boerne, TX, USA); bicinchoninic acid assay protein kit from Pierce Biotechnology, Inc. (Rockford, IL, USA); organic solvents were from J. T. Baker (Phillipsburg, NJ, USA); TRIzol reagent and SuperScript II from Invitrogen (Carlsbad, CA, USA); RNeasy kit from QIAGEN (Valencia, CA, USA); SyBr green Taqman PCR kit from Applied Biosystems (Foster City, CA, USA); Odyssey blocking buffer, goat anti-mouse IgG-IRDye 800, donkey anti-goat IgG-IRDye 680, and goat anti-rabbit IgG-IRDye 680 from Li-Cor Biosciences (Lincoln, NE, USA); corticosterone ELISA kit from Cayman Chemical (Ann Arbor, Michigan, USA); progesterone and testosterone ELISA kits from ALPCO (Salem, NH, USA); pregnant mare’s serum gonadotropin (PMSG)/equine chorionic gonadotropin (eCG), and Bt₂cAMP from Sigma (St. Louis, MO, USA); ³H₂O and ³H-palmitic acid were from PerkinElmer (Waltham, MA, USA). Cortrosyn was from Amphastar Pharmaceuticals Inc. (Rancho Cucamonga, CA, USA).

Hao X., Zhu B., Yang P., Dong D., Sahbaie P., Oliver P.L., Shen W.J., Azhar S, & Kraemer F.B. (2021). SNAP25 mutation disrupts metabolic homeostasis, steroid hormone production and central neurobehavior. Biochimica et biophysica acta. Molecular basis of disease, 1868(2), 166304.

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Lipidomic and Chemerin Expression Analysis

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All chemicals were obtained from Sigma-Aldrich (St. Louis, MO) unless otherwise specified. Cholesterol LiquiColor Test (Enzymatic) was from Stanbio (Boerne, TX, USA); serum non-esterified fatty acid kit from Fujifilm Wako Diagnostic USA (Mountain View, CA); serum glycerol kit from Sigma-Aldrich (St. Louis, MO); bicinchoninic acid assay protein kit from Pierce Biotechnology, Inc (Rockford, IL, USA); organic solvents from J. T. Baker (Phillipsburg, NJ, USA); TRIzol reagent and SuperScript II from Invitrogen (Carlsbad, CA, USA); RNeasy kit from QIAGEN (Valencia, CA, USA); SyBr green Taqman PCR kit from Applied Biosystems (Foster City, CA, USA); M-040274-01-0005 siGENOME siRNA for mouse Rarres2 (71660) from Dharmacon, Inc, (Chicago, IL, USA); mouse chemerin monoclonal antibody (R and D Systems Cat# MAB2325, RRID: AB_2238042), biotinylated anti mouse chemerin polyclonal goat IgG (R and D Systems Cat# BAM2325, RRID: AB_1964540), and recombinant mouse chemerin protein from R&D systems, Inc (Minneapolis, MN, USA); Bio-Rad protein assay dye reagent concentrate from Bio-Rad Laboratories, Inc (Hercules, CA, USA).

Zhang Y., Shen W.J., Qiu S., Yang P., Dempsey G., Zhao L., Zhou Q., Hao X., Dong D., Stahl A., Kraemer F.B., Leung L.L, & Morser J. (2021). Chemerin regulates formation and function of brown adipose tissue: Ablation results in increased insulin resistance with high fat challenge and aging. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(7), e21687.

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