THP1-Blue™ NF-kB cells (InvivoGen, San Diego, CA, USA) were grown in RPMI 1640 supplemented with 10% (v/v) FBS, 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 25 mM HEPES, 100 μg/ml Normocin™, 10 μg/ml Blasticidin. THP1-Blue™ NF-kB cells were treated with TNFα or infected with Pru∆ku80 WT or Δteegr strains and then followed by determination of NF-kB activity using a reporter gene assay. QUANTI-Blue™ (InvivoGen) was added to cells, plates were incubated for a further 2 h and the optical density at 655 nm was recorded. Graphs show results from averaged technical replicates and at least three independent experiments.
Thp1 blue nf kb cells
Manufactured by InvivoGen
Sourced in United States
The THP1-Blue™ NF-kB cells are a reporter cell line derived from the human monocytic THP-1 cell line. These cells stably express an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using thp1 blue nf kb cells
1
Measuring NF-kB Activation in THP1 Cells
2
Measuring NF-kB Activation in THP1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
THP1-Blue™ NF-kB cells (InvivoGen, San Diego, CA, USA) were grown in RPMI 1640 supplemented with 10% (v/v) FBS, 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 25 mM HEPES, 100 μg/ml Normocin™, 10 μg/ml Blasticidin. THP1-Blue™ NF-kB cells were treated with TNFα or infected with Pru∆ku80 WT or Δteegr strains and then followed by determination of NF-kB activity using a reporter gene assay. QUANTI-Blue™ (InvivoGen) was added to cells, plates were incubated for a further 2 h and the optical density at 655 nm was recorded. Graphs show results from averaged technical replicates and at least three independent experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!