High fidelity taq dna polymerase

M-199, RPMI-1640, yeast peptone dextrose medium, Giemsa dye, Trypan blue, MTT, 2′,7′-dichlorodihydrofluorescein diacetate dye, N-acetyl l-cysteine (NAC), R-mevalonate lithium salt, phosphoenolpyruvate, lactate dehydrogenase, pyruvate kinase, β-NADH, ATP, glycine, diphenyleneiodonium chloride, ammonium bicarbonate, urea, sodium chloride, magnesium chloride, manganese chloride, zinc chloride, calcium chloride, copper sulphate, proteinase K, ergosterol (ERG), diphenyl hexatriene (DPH), RNase A, TRIZOL, nitro blue tetrazolium, the lactate dehydrogenase (LDH) assay kit, caprylic acid, 8-amino caprylic acid, 4-methyl octanoic acid, methyl octanoate, valproic acid, the Apoptosis Detection Kit, Taq Polymerase for PCR, Anti-His primary antibody and IgG-HRP conjugated secondary antibody and all solvents were from Sigma-Aldrich Co. (St Louis, MO, USA). The QIAamp DNA Mini kit, RNeasy Mini Kit, High fidelity Taq DNA Polymerase, and Ni-NTA agarose were from Qiagen. from Thermo-Fisher. The cDNA synthesis kit was from Hoffmann-La Roche (Basel, Switzerland). Trypsin, iodoacetamide, and DTT, restriction enzymes like BamHI, HindIII, and XhoI were purchased from Thermo Fischer (Waltham, MA, United States). The RAW 264.1 and THP-1 cell line was obtained commercially from the National Cell Repository, NCCS, Pune.

DNA isolated from the peripheral blood mononuclear cells of infected patients was used to amplify the sequence spanning the Vpu gene by polymerase chain reaction (PCR), using gene-specific primers.^{15 (link)} PCR was performed with High-fidelity Taq DNA polymerase (Qiagen, Germany) using the following primer pair.
Forward primer Vpu 5′-GGCGAATTCTTATGCAACCTATAATAGTAGCAATAGTAGC-3′
Reverse primer Vpu 5′-GGCGTCGACCTACAGATCATCAATATCCCAAGGAG-3′.