Osteoblastic induction was performed using StemProTM Osteogenesis Differentiation Kit (Gibco, Carlsbad, CA, USA). In brief, hPDLSCs were washed with PBS and seeded onto 12-well plates, then cultured with MSC Growth Medium at 37°C in a humidified atmosphere of 5% CO2 for 5 days. Afterwards, culture medium was replaced by Complete Osteogenesis Differentiation Medium (Invitrogen, Carlsbad, CA, USA) and PDLSCs would differentiate to osteoblasts.
Complete osteogenesis differentiation medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Complete Osteogenesis Differentiation Medium is a cell culture medium formulated to support the differentiation of cells into osteoblasts, the cells responsible for bone formation. The medium provides the necessary components to promote the development of a mature osteogenic phenotype in vitro.
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Lab products found in correlation
2 protocols using complete osteogenesis differentiation medium
1
Osteoblastic Induction of hPDLSCs
2
Osteoblastic Induction via StemPro Differentiation
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A StemProTM Osteogenesis Differentiation Kit (Gibco, Waltham, MA, USA) was used to measure osteoblastic induction. The cells were washed and plated in 12-well plates in mesenchymal stem cell growth medium and cultured for 5 days under standard conditions. The medium was then exchanged with complete osteogenesis differentiation medium (Invitrogen).
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