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Complete osteogenesis differentiation medium

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Complete Osteogenesis Differentiation Medium is a cell culture medium formulated to support the differentiation of cells into osteoblasts, the cells responsible for bone formation. The medium provides the necessary components to promote the development of a mature osteogenic phenotype in vitro.

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2 protocols using complete osteogenesis differentiation medium

1

Osteoblastic Induction of hPDLSCs

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Osteoblastic induction was performed using StemProTM Osteogenesis Differentiation Kit (Gibco, Carlsbad, CA, USA). In brief, hPDLSCs were washed with PBS and seeded onto 12-well plates, then cultured with MSC Growth Medium at 37°C in a humidified atmosphere of 5% CO2 for 5 days. Afterwards, culture medium was replaced by Complete Osteogenesis Differentiation Medium (Invitrogen, Carlsbad, CA, USA) and PDLSCs would differentiate to osteoblasts.
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2

Osteoblastic Induction via StemPro Differentiation

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A StemProTM Osteogenesis Differentiation Kit (Gibco, Waltham, MA, USA) was used to measure osteoblastic induction. The cells were washed and plated in 12-well plates in mesenchymal stem cell growth medium and cultured for 5 days under standard conditions. The medium was then exchanged with complete osteogenesis differentiation medium (Invitrogen).
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