Anti mouse igg hrp

Specific details regarding protein extraction were described in our published paper [21 (link)]. The primary detection antibodies used in our experiment were anti-beta-actin (1:1000, Proteintech, Rosemont, IL, USA), anti-Sirt7 (1:1000, Santa, Dallas, TX, USA), anti-alkaline phosphatase (ALP) (1:1000, ABclonal, Wuhan, China), anti-MCP-1 (1:1000, ABclonal, Wuhan, China), anti-Nrf2 (1:1000, Proteintech, Rosemont, IL, USA), anti-NQO1 (1:1000, Proteintech, USA), anti-Hmox1 (1:1000, Proteintech, Rosemont, IL, USA), anti-ICAM-1 (1:1000, Abcam, Waltham, MA, USA), anti-BAX (1:1000, CST, Boston, MA, USA), anti-runt-related transcription factor 2 (Runx2) (1:1000, Proteintech, Rosemont, IL USA) and anti-cleaved caspase3 (1:1000, CST, Boston, MA, USA). The secondary antibodies used were goat anti-rabbit and anti-mouse IgG-HRP (1:5000, Fudebio, Hangzhou, China). The quantitation of Immunoblotting images was analyzed using ImageJ software (NIH, Bethesda, MA, USA).

Proteins were extracted, quantified and analyzed as reported previously (Xiong et al. 2018 (link); Liu et al. 2019 (link); An et al. 2021 (link)). The primary and secondary antibodies used included anti-β-MHC, anti-ANP, anti-HO1, anti-GPX4 (1:1000, Proteintech, USA), anti-PINK1 (1:200, Santa Cruz, USA), anti-SIRT1 (1:1000, Cell Signaling Technology, USA), goat anti-rabbit and anti-mouse IgG-HRP (1:5000, Fudebio, China) antibodies.