Skin tissue pieces from an adult TEX11_1258Ins(TT) male mouse were used to derive fibroblasts. At passage 2, these fibroblasts were transduced with non-integrated CytoTune iPS 2.0 Sendai Reprograming Kit (ThermoFisher). Seven days post-induction with Sendai viruses, putative iPSCs (MWRIi001-A-3) were seeded on mitomycin-C treated mouse embryonic fibroblasts (MEF) in GlutaMAX DMEM/F12 (ThermoFisher), 20% Knockout Serum Replacement-Mutiple Species (ThermoFisher), 1% NEAA, 1% 2-Methylmethacathinone (ThermoFisher), and 1ug mouse Leukemia Inhibitory Factor (Invitrogen). iPSCs were cultured in 37 °C, 5%CO2, and passaged using TrypLE (ThermoFisher) as single cells every 3–5 days.
Knockout serum replacement mutiple species
Manufactured by Thermo Fisher Scientific
Knockout Serum Replacement-Mutiple Species is a laboratory reagent designed to support the growth and maintenance of a variety of cell lines. It is a proprietary serum-free formulation that can be used as a replacement for fetal bovine serum (FBS) in cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
2 methylmethacathinone, by Thermo Fisher Scientific (2 mentions)
Dmem f 12 glutamax, by Thermo Fisher Scientific (2 mentions)
Mouse leukemia inhibitory factor, by Thermo Fisher Scientific (2 mentions)
Cytotune ips 2.0 sendai reprograming kit, by Thermo Fisher Scientific (2 mentions)
Tryple, by Thermo Fisher Scientific (2 mentions)
2 protocols using knockout serum replacement mutiple species
1
Generation of Mouse Fibroblast-Derived iPSCs
2
Generation of Mouse Fibroblast-Derived iPSCs
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Skin tissue pieces from an adult TEX11_1258Ins(TT) male mouse were used to derive fibroblasts. At passage 2, these fibroblasts were transduced with non-integrated CytoTune iPS 2.0 Sendai Reprograming Kit (ThermoFisher). Seven days post-induction with Sendai viruses, putative iPSCs (MWRIi001-A-3) were seeded on mitomycin-C treated mouse embryonic fibroblasts (MEF) in GlutaMAX DMEM/F12 (ThermoFisher), 20% Knockout Serum Replacement-Mutiple Species (ThermoFisher), 1% NEAA, 1% 2-Methylmethacathinone (ThermoFisher), and 1ug mouse Leukemia Inhibitory Factor (Invitrogen). iPSCs were cultured in 37 °C, 5%CO2, and passaged using TrypLE (ThermoFisher) as single cells every 3–5 days.
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