Peroxidase labeled horse anti mouse igg

Primary antibodies used in this study for Western blot analysis included a well-characterized homemade rabbit antiserum against the last 17 amino acids of APP, named APP-Cter-C17 (1/5000) [11 (link),12 (link),37 (link)], LC3B obtained from Cell Signaling (1/1000), p62 (Abcam, 1/2000, Cambridge, GB), and α-tubulin (Sigma, 1/10,000). The anti-histone H3 (1/10,000) used for normalization was obtained from Sigma (Saint-Louis, MO, USA). Secondary antibodies (peroxidase-labeled goat anti-rabbit IgG, 1/5000 or peroxidase-labeled horse anti-mouse IgG, 1/50,000) were obtained from Vector Laboratories (Eurobio Scientific, Les Ulis, France).

Anti clathrin heavy chain antibody (BF-06, Thermo Scientific Pierce, Rockford, IL) was used as the primary antibody in immunofluorescence assays. Mouse monoclonal antibodies to gB (2F12), pUL44 (ICP36), and IE1 (CH160) were purchased from Virusys corporation, Sykesville, MD, USA. Fluorescent label tagged secondary antibody DYLIGHT 594 was purchased from Thermo Scientific Pierce and used in immunofluorescent assays (IFA) described below. Hoechst 33258 (Thermo Scientific Pierce) staining identified the nuclei in IFA. Anti β-actin antibody (AC-74, Sigma-Aldrich, St Louis, Mo, USA) was used as a control for sample loading in immunoblots. Peroxidase-labeled horse anti-mouse IgG (Vector Laboratories, Burlingame, CA) was used as the secondary antibody for IBs. Blots were detected using ECL Western blotting detection reagents (GE Healthcare, Buckinghamshire, United Kingdom).