An inverted microscope (Axiovert 100; Carl Zeiss) equipped with an interline cooled CCD camera (Princeton Instruments MicroMAX; Roper Scientific) was used for the recording of epifluorescence images of oocytes incubated in M2 medium at 37 °C. GFP-tagged Geminin was imaged using a filter set comprised of an excitation filter with a band pass of 450–490 nm, a dichroic mirror 510 nm and a band pass 505–520 nm emission filter. A DAPI (4,6-diamidino-2-phenylindole) filter set was used for imaging Hoechst 33342 (excitation filter: 340–380 nm, dichroic mirror: 400–420 nm, emission filter: 435–485 nm). Oocytes were imaged every 15 min to minimize photobleaching and photodamage. Data were collected and analysed using MetaMorph and Meta-Fluor software 6.1 (Molecular Devices).
Princeton instruments micromax
Manufactured by Teledyne
The Princeton Instruments MicroMAX is a compact and versatile scientific imaging camera designed for a wide range of microscopy and spectroscopy applications. It features a high-sensitivity, low-noise CCD sensor and advanced electronics for high-quality image capture. The MicroMAX offers flexible connectivity options and can be easily integrated into various experimental setups.
Automatically generated - may contain errors
Lab products found in correlation
Meta fluor software 6, by Molecular Devices (2 mentions)
Axiovert 100, by Zeiss (2 mentions)
Metamorph, by Molecular Devices (2 mentions)
Electrophysiological amplifier, by World Precision Instruments (1 mentions)
Pneumatic picopump, by World Precision Instruments (1 mentions)
Dm irb, by Leica camera (1 mentions)
2 protocols using princeton instruments micromax
1
Fluorescent Imaging of Oocyte Dynamics
2
Oocyte Microinjection and Fluorescence Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All microinjections of GV stage oocytes were performed in M2 medium on the stage of an inverted microscope (DM IRB; Leica). In brief, fabricated micropipettes were inserted into cells using the negative capacitance overcompensation facility on an electrophysiological amplifier (World Precision Instruments), whereas cells were immobilized with a holding pipette (Hunter Scientific). A precise injection volume (2–5% of the total egg volume) was achieved using a pressure regulator (Pneumatic PicoPump; World Precision Instruments). Epifluorescence images of oocytes incubated in M2 medium at 37°C were recorded using an objective (20×, 0.75 NA) on an inverted microscope (Axiovert 100; Carl Zeiss) equipped with an interline cooled charge-coupled device camera (Princeton Instruments MicroMAX; Roper Scientific). GFP-tagged securin and geminin were imaged using an FITC filter set at band pass 450–490 nm for excitation, dichroic mirror 510 nm, and band pass 520 nm for emission. Oocytes were imaged every 10 min to minimize photobleaching and photodamage. MetaMorph and MetaFluor software 6.1 (Molecular Devices) were used for image capture and data analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!