Cells were incubated with FC-block (BD Biosciences) for 10 min and surface staining was performed at 4°C for 15 min. Cells were run on LSRII flow cytometer (BD Biosciences) and data were analyzed by FlowJo (Tristar). For lipid staining by flow cyometry, cells were re-suspended in 500 μl of Bodipy 493/503 at 0.25 μg/ml in PBS. Cells were stained for 15 min at room temperature in the dark, then washed twice, re-suspended in PBS and run immediately on LSRII. For lipid staining by confocal microscopy, cells were washed twice with PBS, resuspended in complete RPMI and 50,000 cells were seeded on poly-L-lysine cellware 12 MM round coverslips (Corning) for 45 min at 37 °C. Cells were fixed and permeabilized with Fixation & Permeabilization Buffers (BD Biosciences) for 15 min at RT, washed twice with wash buffer (BD Biosciences) and then stained with BODIPY for 15 min at RT. Cells were washed and incubated with DAPI and mounted on slides using Prolong Gold antifade reagent (Life Technology). The cells were imaged with a Leica TCS SP5 laser scanning confocal microscope (Leica Microsystems).
Poly l lysine cellware 12 mm round coverslips
Manufactured by Corning
Poly-L-lysine cellware 12 MM round coverslips are a type of laboratory equipment used to support cell culture. They are composed of round coverslips coated with the chemical compound poly-L-lysine, which helps promote the attachment and growth of cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using poly l lysine cellware 12 mm round coverslips
1
Lipid Staining in Cells by Flow Cytometry and Confocal Microscopy
2
Lipid Staining in Cells by Flow Cytometry and Confocal Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were incubated with FC-block (BD Biosciences) for 10 min and surface staining was performed at 4°C for 15 min. Cells were run on LSRII flow cytometer (BD Biosciences) and data were analyzed by FlowJo (Tristar). For lipid staining by flow cyometry, cells were re-suspended in 500 μl of Bodipy 493/503 at 0.25 μg/ml in PBS. Cells were stained for 15 min at room temperature in the dark, then washed twice, re-suspended in PBS and run immediately on LSRII. For lipid staining by confocal microscopy, cells were washed twice with PBS, resuspended in complete RPMI and 50,000 cells were seeded on poly-L-lysine cellware 12 MM round coverslips (Corning) for 45 min at 37 °C. Cells were fixed and permeabilized with Fixation & Permeabilization Buffers (BD Biosciences) for 15 min at RT, washed twice with wash buffer (BD Biosciences) and then stained with BODIPY for 15 min at RT. Cells were washed and incubated with DAPI and mounted on slides using Prolong Gold antifade reagent (Life Technology). The cells were imaged with a Leica TCS SP5 laser scanning confocal microscope (Leica Microsystems).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!