Proteins were obtained from the hippocampal tissue or cultured neurons or isolated mitochondria. For western blotting, 35–50 μg protein was added per lane of 12% SDS-PAGE. Primary antibodies were diluted in primary antibody dilution buffer (Beyotime Biotech., Shanghai, China). Antibodies used included the following: anti-GAPDH (Abgent Biotech. Co. Ltd., Suzhou, China), anti-ferritin, superoxide dismutase 2 (SOD2), Mitoferrin1, Drp1, Mfn2, RASD1, NMDAR1, and NMDAR2A (Abcam, Cambridge, MA, USA), anti-TfR1 (Zymed, San Francisco, CA, USA), anti-IRP2 (polyclonal, raised from rabbit), and anti-DMT1 (Alpha Diagnostic International, San Antonio, TX, USA). Detection was performed using peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, UK). Quantification of the density of the western bands was done with programme ImageJ (http://rsb.info.nih.gov/ij/ ).
Anti dmt1
Manufactured by Alpha Diagnostic
Sourced in United States
The Anti-DMT1 is a lab equipment used to detect and quantify the presence of the Divalent Metal Transporter 1 (DMT1) protein in biological samples. It functions as an analytical tool to measure the expression levels of this protein, which is involved in the transport of various divalent metal ions across cell membranes.
Automatically generated - may contain errors
Lab products found in correlation
Primary antibody dilution buffer, by Beyotime (2 mentions)
Nmdar2a, by Abcam (2 mentions)
Anti ferritin, by Abcam (2 mentions)
Nmdar1, by Abcam (2 mentions)
Rasd1, by Abcam (2 mentions)
Superoxide dismutase 2 sod2, by Abcam (2 mentions)
Mitoferrin1, by Abcam (2 mentions)
Anti irp2, by Alpha Diagnostic (2 mentions)
Peroxidase conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
Anti tfr1, by Zymo Research (2 mentions)
2 protocols using anti dmt1
1
Quantitative Protein Analysis of Hippocampal Samples
2
Protein Analysis in Hippocampus and Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Proteins were obtained from hippocampal tissue or cultured neurons or isolated mitochondria. For western blotting, 35-50 µg protein was added per lane of 12% SDS-PAGE. Primary antibodies were diluted in primary antibody dilution buffer (Beyotime Biotech., Shanghai, China). Antibodies used included: anti-GAPDH (Abgent Biotech. Co. Ltd., Suzhou, China), anti-ferritin, superoxide dismutase 2 (SOD2), Mitoferrin1, Drp1, Mfn2, RASD1, NMDAR1, and NMDAR2A (Abcam, Cambridge, MA, USA), anti-TfR1 (Zymed, San Francisco, CA, USA), anti-IRP2 (polyclonal, raised from rabbit), and anti-DMT1 (Alpha Diagnostic International, San Antonio, TX, USA). Detection was performed using peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, UK). Quantification of the density of the western bands was done with programme ImageJ (http://rsb.info.nih.gov/ij/).
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