Protein isolation and Western blot analyses were performed according to previously published methods [7 (link)]. Primary antibodies were obtained from the following sources and used at the following dilutions: LC3B rabbit polyclonal (#2775S, 1:1000), p-ERK1/2 rabbit polyclonal (#9101S, 1:1000), total MAPK rabbit polyclonal (#4695S, 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit polyclonal (#2118S, 1:4000), and FTH1 rabbit polyclonal (#3998S, 1:1000) were obtained from Cell Signaling Technology. CD71 mouse monoclonal (2B6, sc-51829, 1:100), TOM20 rabbit polyclonal (FL-145, sc-11415, 1:7500), TOM70 mouse monoclonal (A-8, sc-390545, 1:1000), IRP1 goat polyclonal (N-17, sc-14216, 1:1000) and IRP2 mouse monoclonal (7H6, sc-33682, 1:500) were obtained from Santa Cruz Biotechnology.
P erk1 2 rabbit polyclonal
Manufactured by Cell Signaling Technology
P-ERK1/2 rabbit polyclonal is a lab equipment product that detects phosphorylated forms of ERK1 and ERK2 proteins. It is a polyclonal antibody raised in rabbit.
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2 protocols using p erk1 2 rabbit polyclonal
1
Isolation and Immunoblotting of Cellular Proteins
2
Western Blotting Antibody Protocol
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Western blotting was performed as previously described.22 We used the antibodies according to the manufacturers' instructions. The primary antibodies were as follows: β‐actin mouse monoclonal (60008‐1‐Ig), SPON2 rabbit polyclonal (20513‐1‐AP), ERK1/2 rabbit polyclonal (16443‐1‐AP), JNK rabbit polyclonal antibody (51151‐1‐AP), p38 rabbit polyclonal (14064‐1‐AP), vimentin rabbit polyclonal (10366‐1‐AP), N‐cadherin rabbit polyclonal (22018‐1‐AP), E‐cadherin rabbit polyclonal (20874‐1‐AP; all from Proteintech Group, Inc), BAX rabbit polyclonal (#5023), BCL‐2 rabbit polyclonal (#9942), cleaved caspase‐3 rabbit polyclonal (#9664), cleaved PARP mouse polyclonal (#5625), p‐ERK1/2 rabbit polyclonal (#4370; all from Cell Signaling Technology), p‐p38 (D‐8; sc‐7973) and p‐JNK antibody (G‐7; sc‐6254; both from Santa Cruz Biotechnology, Inc).
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