Alexa fluor 546 red goat anti rabbit antibody

Cells were placed on coverslips in 24-well plates and then washed with cold PBS three times. Then, the coverslips were fixed with 4% formaldehyde and immunostained using monoclonal antibodies of E-cadherin and Vimentin. After that, DNA of these cells was stained with 4′,6-diamidino-2-phenylindole (DAPI). The Alexa Fluor 546 (red) goat anti-rabbit antibody (Molecular Probes, OR, USA) was used for E-cadherin (no. 14472, 1:50; Cell Signaling Technology, Boston, MA, USA) and Vimentin (sc-66002, 1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA). Immunofluorescence was determined using confocal microscopy (Leica TCS SP5).

Cells were seeded on coverslips in 24-well plates, then washed in cold PBS three times, fixed with 4% paraformaldehyde, and immunostained for NIP30 or REGγ or GFP, as well as DNA staining with 4, 6-diamidino-2-penylindole (DAPI). Then Alexa Fluor 546 (red) goat anti-rabbit antibody (Molecular Probes, OR) was added. Immunofluorescence was visualized by Fluorescence microscopy (Leica).