Routine histopathological processing, including immunohistochemistry (IHC), of the patients’ tumor tissues was performed at the Department of Pathology of the Medical University of Vienna. The antibody panels used for immunohistochemistry included bcl-2 (clone 124; DAKO Agilent, Santa Clara, CA, USA), CD34 (clone QBend/10; Leica, Wetzlar, Germany), CD99 (clone EP8, Biocare, Pacheco, CA, USA), STAT6 (anti-Stat 6 polyclonal antibody; Sigma-Aldrich, St. Louis, MO, USA), and Ki67 (clone 30-9; Ventana, Oro Valley, AZ, USA). In addition, all cases were re-reviewed by the author LM to confirm pathologic SFT diagnosis.
Cd34 clone qbend 10
Manufactured by Leica
Sourced in United States, United Kingdom
CD34 (clone QBend/10) is a laboratory reagent used for the identification and enumeration of CD34-positive cells in biological samples. It is a monoclonal antibody that specifically binds to the CD34 antigen, which is expressed on the surface of hematopoietic stem and progenitor cells.
Automatically generated - may contain errors
2 protocols using cd34 clone qbend 10
1
Routine Histopathological SFT Analysis
2
Immunohistochemical Analysis of Splenic Stroma
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Splenic stroma and red pulp vessels were immunohistochemically studied with primary antibodies against the following antigens: (i) CD34 (clone QBEnd/10, Leica; Newcastle, United Kingdom); (ii) CD8 (clone C8/144B, Dako; Glostrup, Denmark); (iii) Smooth Muscle Actin (SMA, clone 1A4, Dako); (vi) CD68 (clone PGM1, Dako). Antigen detection was performed using an automated immunostainer (Bond-maX; Leica, Newcastle Upon Tyne, UK), as previously described (15) . In SCD and HS cases, the number of SMA-positive stromal cells was assessed by comparison with control spleens and graded as increased, normal, or reduced.
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