Fuji dri chem clinical chemistry analyzer fdc 3500

We collected blood (~500 μL) by facial vein puncture at the time of tissue sampling at around CT20. Serum was separated by centrifugation at 3000 rpm for 5 min and was sent to Taiwan Animal Consortium (Taipei, Taiwan) for biochemical analyses. Analyses were performed using Fuji Dri-Chem Clinical Chemistry Analyzer FDC 3500 (FujiFilm, Tokyo, Japan), which uses colorimetry and electrolyte measurement (https://www.fujifilm.eu/fileadmin/migration_uploads/FUJI_DRI-CHEM4000i.pdf). Blood urea nitrogen (BUN) level that exceeded measurement precision was indicated “>140 mg/dl” in the analysis report and this upper cap was used as-is in statistical analysis since the value is much beyond the normal range. Compared to enzymatic or high-performance liquid chromatography (HPLC) methods, the colorimetric method used in this study can overestimate the absolute concentration of creatinine (CRE) [46 (link)]. We have therefore used estimated CRE as a relative measure only.

Serum samples were collected at week 12, and biochemical nutritional markers TC, TG, HDL, alanine transaminase (ALT), aspartate transaminase (AST), and inflammatory cytokines (IL-1β and IL-10) were measured. Biochemical, and nutritional markers were measured using a Fuji DRI-CHEM Clinical Chemistry Analyzer FDC 3500 (Fujifilm, Tokyo, Japan), and LDL level was calculated using the Friedewald formula^{42 (link)}. Serum inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA) kits (IL-1β, Abcam, ab197742; IL-10, Abcam, ab255729). Experiments were performed in accordance with the manufacturer’s instructions. Optical densities were measured at 450 nm using a spectrophotometer (SpectraMax ABS Plus, San Jose, CA, USA).

The serum samples were collected from test mice and stored at −80 °C until analysis. Serum glucose, triglyceride (TG) and total cholesterol (T-Chol) levels were measured using a Fuji DRI-CHEM Clinical Chemistry Analyzer FDC 3500 (Fujifilm, Tokyo, Japan) at the National Laboratory Animal Center (Taipei, Taiwan). Serum insulin level was measured using a mouse insulin assay kit following the manufacturer’s protocol (Mercodia, Uppsala, Sweden). Homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated according to the formula: HOMA-IR = insulin (μU/mL) × glucose (mg/dL)/405. Serum peptide YY (PYY), pancreatic polypeptide (PP) and resistin levels were measured using the Milliplex® MAP Kit and Mouse Metabolic Hormone 96-Well Plate Assay (Merck Millipore, MA, USA).