H 2kb pe

Manufactured by BioLegend

H-2Kb PE is a flow cytometry antibody used for the detection and quantification of H-2Kb, a major histocompatibility complex (MHC) class I molecule, in mouse cells. It is conjugated with the fluorescent dye Phycoerythrin (PE).

Automatically generated - may contain errors

Lab products found in correlation

70 μm mesh, by BD (1 mentions) Cd69 bv421, by BioLegend (1 mentions) Bv510 tcrβ, by BioLegend (1 mentions) Cd4 buv395, by BD (1 mentions) Cd3 buv737, by BD (1 mentions) Ccr7 pe cy7, by BioLegend (1 mentions) Cd8 percp cy5, by BioLegend (1 mentions) Siinfekl, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-H-2Kb- SIINFEKL-PE PE anti-mouse H-2Kb/H-2Db PE anti-mouse H-2Kb Anti-H-2Kb PE PE anti-mouse SIINFEKL-H-2Kb antibody H-2Kb

2 protocols using h 2kb pe

Flow Cytometric Analysis of Thymocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thymuses from WT or Yap-cKO mice (8–10 weeks old) were mechanically disrupted by being pushed through a 70 μm mesh (Falcon) with an insulin syringe plunger and washed with PBS. Cells were treated with ACK red blood cell lysis buffer, and thymocyte single-cell suspensions were stained for analysis by flow cytometry as described earlier. Cells were stained with dead cell dye and antibodies for the following surface markers: CD3 BUV737 (BD), CD4 BUV395 (BD), CD8 PerCP-Cy5.5 (Biolegend), TCRβ BV510 (Biolegend), CCR7 PECy7 (Biolegend), H-2Kb PE (Biolegend), CD69 BV421 (Biolegend), CD45R/B220 APCFire750 (Biolegend), CD25 APCFire750 (Biolegend), GL3 APCFire750 (Biolegend), and NK1.1 APCFire750 (Biolegend). Thymocytes were also stained intracellularly with anti-Nur77 APC (BD) using the eBioscience Foxp3/Transcription factor staining buffer set, as described earlier.

Stampouloglou E., Cheng N., Federico A., Slaby E., Monti S., Szeto G.L, & Varelas X. (2020). Yap suppresses T-cell function and infiltration in the tumor microenvironment. PLoS Biology, 18(1), e3000591.

+ Open protocol

+ Expand

Murine B-Cell APC Function Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine B_Vax were tested for the expression of molecules associated with the APC function by flow cytometry. Cells were stained with the following anti-mouse Abs (all from BioLegend unless otherwise specified): IA^b PerCP-eFluor 710 (AF6-120.1), H-2K^b PE (AF6-88.5.5.3), CD86 AF700 (GL1), and 4-1BBL PerCP-Cy5.5 (5F4). After pulsing with 100 ng/ml SIINFEKL (Sigma-Aldrich), the peptide presentation via H-2K^b was assessed using the anti-mouse SIINFEKL–H-2K^b PE-Cy7 (eBio25-D1.16; eBioscience).

Lee-Chang C., Miska J., Hou D., Rashidi A., Zhang P., Burga R.A., Jusué-Torres I., Xiao T., Arrieta V.A., Zhang D.Y., Lopez-Rosas A., Han Y., Sonabend A.M., Horbinski C.M., Stupp R., Balyasnikova I.V, & Lesniak M.S. (2020). Activation of 4-1BBL+ B cells with CD40 agonism and IFNγ elicits potent immunity against glioblastoma. The Journal of Experimental Medicine, 218(1), e20200913.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!