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Recombinant human follistatin

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Recombinant human follistatin is a protein produced using recombinant DNA technology. It is a secreted glycoprotein that primarily functions as a binding protein for activin, a member of the transforming growth factor-beta (TGF-β) superfamily.

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Follistatin (28 citations)

2 protocols using recombinant human follistatin

1

Follistatin Modulation of Embryo Development

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Experiment was performed as described in Fig. 1. After IVM and IVF, presumptive zygotes were cultured with 0 or 10 ng/ml recombinant human follistatin (R&D Systems, Minneapolis, MN) [4 (link)] in the presence or absence of AKT inhibitor III (75 μM) or AKT inhibitor IV (3.5 μM) until 72 hpi (n = 4 replicates/inhibitor, n = 20–30 zygotes/group). Then, 8- to 16-cell stage embryos from each group were separated and cultured in fresh KSOM medium supplemented with 0.3% BSA and 10% FBS without any treatment until d 7. Effects of exogenous follistatin on early and total cleavage, development to 8–16-cell and d7 blastocyst rates were recorded.
Ashry M., Rajput S.K., Folger J.K., Knott J.G., Hemeida N.A., Kandil O.M., Ragab R.S, & Smith G.W. (2018). Functional role of AKT signaling in bovine early embryonic development: potential link to embryotrophic actions of follistatin. Reproductive Biology and Endocrinology : RB&E, 16, 1.
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2

Follistatin Effects on Bovine Embryos

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Ovaries collection, oocytes retrieval, and in vitro embryo production were performed as previously described (Bettegowda, Patel, Ireland, & Smith, 2006 (link)). Zygotes were cultured in the presence or absence of 10 ng/ml recombinant human follistatin (R&D Systems, Minneapolis, MN) during the initial 72 hr of in vitro culture (Lee et al., 2009 (link)). Then, 8–16 cell embryos were isolated, washed, and cultured in fresh media without follistatin until Day 7 when blastocysts were collected and snap-frozen in lysis buffer and stored at −80°C until used for nucleic acids isolation.
Ashry M., Rajput S.K., Folger J.K., Yang C., Knott J.G, & Smith G.W. (2020). Follistatin treatment modifies DNA methylation of the CDX2 gene in bovine preimplantation embryos. Molecular reproduction and development, 87(9), 998-1008.
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