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2 protocols using ammonium persulfate aps

1

Synthesis and Characterization of Multifunctional Polymer Conjugates

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Tetra‐poly(ethyl glycol)‐amine (Sunbright PTE‐050PA; Mn, 5328 g/mol) was purchased from NOF Corporation (Tokyo, Japan). N,N,N′,N′‐tetramethylethylenediamine (TEMED), dichloromethane, acryloyl chloride, triethylamine, ammonium persulfate (APS), hydrochloric acid, methanol, and diethyl ether were purchased from Wako Pure Chemical Industries (Osaka, Japan). Dimethyl sulfoxide, 2‐morpholineoethanesulfonic acid and 4‐dimethylaminopyridine (DMAP) were obtained from Nacalai Tesque (Kyoto, Japan). Dextran with a weight‐averaged molecular weight of 40 000 (Dex), diethylenetriaminepentaacetic acid (DTPA) anhydride, manganese chloride, first generation polypropylenimine tetramine dendrimer (DAB‐Am‐4), and rhodamine 6G were purchased from Sigma‐Aldrich (St. Louis, Missouri). Water was purified with a Milli‐Q apparatus (Millipore, Bedford, Massachusetts). A detailed materials preparation protocol has been published previously.16, 17, 18
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2

Synthesis and Characterization of Thermoresponsive Hydrogels

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N-Isopropylacrylamide (NIPAAm) was kindly provided by KJ Chemicals (Tokyo, Japan) and purified by recrystallization from toluene/hexane. N-(3-Aminopropyl)methacrylamide hydrochloride (NAPMAm) was purchased from Polyscience (Warrington, PA) and used as received. N,N'-Methylenebisacrylamide (MBAAm), ammonium persulfate (APS), N,N,N′,N′-tetramethylethylenediamine (TEMED), ethyl 2-bromoisobutyrate (EBiB), copper(I) bromide, dimethyl sulfoxide (DMSO) sodium bromate (NaBrO 3 ), 1 M HNO 3 aqueous solution and MA were purchased from Wako Pure Chemical Industries (Osaka, Japan). The water used in this study was purified by a water purifier (WA200, Yamato Scientific, Tokyo, Japan). 2-Bromoisobutanoic acid N-hydroxysuccinimide ester (abbreviated as NHS-initiator) 19 and rhodamine B hydroxyethyl methacrylate ester (RhB) 20 were synthesized according to previous works. Bis (2,2′-bipyridine) (1-(4′-methyl-2,2′-bipyridine-4-carbonyloxy)-2,5-pyrrolidinedione) ruthenium(II) (Ru(bpy) 3 -NHS) was purchased from Trylead Chemical (Hangzhou, China).
Glass capillaries (inner diameter = 850 μm) were purchased from Nichiden Rika Glass Co., Ltd. (Kobe, Japan).
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