Proteins were extracted and visualized using standard techniques as already described [47 (link)]. Specifically, primary anti Chk1 (G4), pT14/Y15-Cdk1, Cdk1, Cdk2, pERK, ERK, p27 and actin were purchased from Santa Cruz Biotechnology. Primary anti pS317-Chk1, pT308-AKT and AKT were purchased from Cell Signaling Technology. Primary anti pY15-Cdk2 was purchased from Abcam. The mouse monoclonal anti-Ran (clone 20) is from BD Transduction Laboratories.
Primary anti ps317 chk1
Manufactured by Cell Signaling Technology
Primary anti pS317-Chk1 is a lab equipment product that detects the phosphorylated form of the Chk1 protein at serine 317. It is used to identify and quantify the levels of this specific post-translational modification in biological samples.
Automatically generated - may contain errors
2 protocols using primary anti ps317 chk1
1
Protein Visualization and Analysis
2
Protein Visualization and Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Proteins were extracted and visualized using standard techniques, and as already described [15 (link)]. Primary anti Chk1 (G4), cyclin D1, Cdt1, actin and Δ-tubulin were purchased from Santa Cruz Biotechnology. Primary anti pS317-Chk1, was purchased from Cell Signaling Technology. The mouse monoclonal anti-cyclin A is from BD Transduction Laboratories. The anti γH2AX antibody is from Millipore.
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