Hippocampus tissues and hippocampal neurons were treated with ice-cold RIPA lysis buffer (Beyotime, Shanghai, China) to extract protein. After determining the protein concentrations using BCA Assay Kit (Beyotime), protein samples were separated in SDS-PAGE gels and then transferred onto PVDF membranes (Invitrogen). Subsequently, the membrane was incubated with skim milk, primary antibody against BDNF (1:2000, Boster, Beijing, China) or GAPDH (1:2000, Boster), and secondary antibody (Goat anti-rabbit, 1:10,000, Boster) in turn. The signal intensity was determined by Super ECL Detection Reagent (Yeasen, Shanghai, China).
Primary antibody against
Manufactured by Boster Bio
Sourced in China
This primary antibody is designed for use in various immunological techniques, such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA). It recognizes a specific target antigen and can be used to detect and quantify the presence of that target in biological samples. The antibody is produced through rigorous quality control and validation processes to ensure its specificity and reliability.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using primary antibody against
1
BDNF Protein Quantification in Hippocampus
2
Quantifying Dopaminergic Neurons in Mouse Midbrain
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The number of dopaminergic neurons was measured by detecting tyrosine hydroxylase (TH) expression in midbrain of mouse. In brief, the midbrain of each mouse was cut into sections to a thickness of 5 μm. The sections were washed using Tris-buffered saline (TBS) and then incubated with TBS+ (0.3% Triton-X100 and 2% bovine serum albumin in TBS) for 45 min. Thereafter, primary antibody against TH (1:500, Boster, Wuhan, China) was used to incubate the sections for 48 h. After being washed by TBS, the sections were incubated with FITC-conjugated secondary antibody (1:500, Boster, Wuhan, China) for 3 h. The TH immunopositive dopaminergic neurons were observed under fluorescence microscope with red fluorescence as positive TH expression signals [41 (link)].
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