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Er1394 monochrome camera

Manufactured by Hamamatsu Photonics
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The ER1394 is a monochrome camera from Hamamatsu Photonics. It utilizes a 1394 FireWire interface for data transfer. The camera is designed to capture high-quality monochrome images.

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Lab products found in correlation

Gram staining kit, by Merck Group (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Axioplan2 upright microscope, by Zeiss (2 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions)

2 protocols using er1394 monochrome camera

1

Gram Staining and Fluorescence Imaging of SWNT-Labeled S. aureus Infection

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5 µm thick tissue sections were mounted on glass slides and stained with Gram staining kit (Sigma-Aldrich, St. Louis, MO). They were observed with a Zeiss Axioplan II upright microscope with objective lenses of 20× (air) and 100× (oil immersion) magnification. Further, in order to determine the localization of the targeted SWNT probe at the site of bacterial infection, we used M13 labeled with a green fluorescent dye, Alexa Fluor 488 (Life Technologies, NY, USA). The anti-S. aureus-M13-SWNT-AlexaFluor488 probe was injected into the infected mice, and after imaging and sacrificing the animals, tissue sections were obtained. The samples were treated for 24hr. with ProLong Gold antifade reagent (Invitrogen Molecular Probes, OR, USA), followed by Gram staining. Fluorescence images were taken using a EXFO-X-cite fluorescence illumination source, and a Cy2 fluorescence filter cube paired with a Hamamatsu Orca ER1394 monochrome camera, with colocalized brightfield images.
Bardhan N.M., Ghosh D, & Belcher A.M. (2014). Carbon Nanotubes as in vivo Bacterial Probes. Nature communications, 5, 4918.
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2

Gram Staining and Fluorescence Imaging of SWNT-Labeled S. aureus Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
5 µm thick tissue sections were mounted on glass slides and stained with Gram staining kit (Sigma-Aldrich, St. Louis, MO). They were observed with a Zeiss Axioplan II upright microscope with objective lenses of 20× (air) and 100× (oil immersion) magnification. Further, in order to determine the localization of the targeted SWNT probe at the site of bacterial infection, we used M13 labeled with a green fluorescent dye, Alexa Fluor 488 (Life Technologies, NY, USA). The anti-S. aureus-M13-SWNT-AlexaFluor488 probe was injected into the infected mice, and after imaging and sacrificing the animals, tissue sections were obtained. The samples were treated for 24hr. with ProLong Gold antifade reagent (Invitrogen Molecular Probes, OR, USA), followed by Gram staining. Fluorescence images were taken using a EXFO-X-cite fluorescence illumination source, and a Cy2 fluorescence filter cube paired with a Hamamatsu Orca ER1394 monochrome camera, with colocalized brightfield images.
Bardhan N.M., Ghosh D, & Belcher A.M. (2014). Carbon Nanotubes as in vivo Bacterial Probes. Nature communications, 5, 4918.
+ Open protocol
+ Expand

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