Serum levels of TNF-α and IL-6 were measured using standard quantitative sandwich ELISA kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol.
Standard quantitative sandwich elisa kits
Manufactured by R&D Systems
Sourced in United States
Standard quantitative sandwich ELISA kits are laboratory tools used to measure the concentration of specific proteins or antigens in a sample. These kits provide a consistent and reliable method for quantitative analysis of target analytes in a variety of sample types.
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3 protocols using standard quantitative sandwich elisa kits
1
Quantification of Serum Inflammatory Markers
2
Renal Dysfunction Biomarkers Measurement
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Plasma creatinine and blood urea nitrogen (BUN) levels were analyzed using a creatinine assay kit (BioAssay Systems, Hayward, CA, USA) and a BUN assay kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively, according to the manufacturer’s protocol. A creatinine value more than 0.5 mg/dL [19 (link)] or a BUN value more than 33 mg/dL [20 (link)] was considered as acute renal failure. Plasma TNF-α and interleukin-6 (IL-6) levels were measured using standard quantitative sandwich ELISA kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. Renal malondialdehyde (MDA) levels were measured using a colorimetric/fluorometric assay kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol. Renal levels of reduced glutathione (GSH) and oxidized glutathione (GSSG) were analyzed using a colorimetric detection kit (Enzo Life Sciences, Farmingdale, NY, USA) according to the manufacturer′s protocol.
3
Quantifying Plasma Cytokine Levels
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Plasma TNF-α and interleukin-6 (IL-6) levels were measured using standard quantitative sandwich ELISA kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol.
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