Phosphate Buffered Saline 1x is a commonly used buffer solution in laboratory applications. It is a balanced salt solution that maintains a stable pH and ionic concentration, providing a physiologically relevant environment for various biological experiments and procedures.
Example 5
DLX2323 was stored in PBS buffer pH 7.2 (Phosphate Buffered Saline 1×, Gibco, LIFE TECHNOLOGIES™, cat. no. 20012). To determine its maximum solubility, DLX2323 was concentrated using Vivaspin 20 centrifuge concentrators (Sartorius Stedim Biotech, cat. no. VS2001) at room temperature. The concentration process was stopped at 71 mg/ml due to the high viscosity of the sample. The obtained DLX2323 protein solution was viscous, clear, and no precipitates were observed by visual inspection.
Example 5
DLX2323 was stored in PBS buffer pH 7.2 (Phosphate Buffered Saline 1×, Gibco, LIFE TECHNOLOGIES™, cat. no. 20012). To determine its maximum solubility, DLX2323 was concentrated using Vivaspin 20 centrifuge concentrators (Sartorius Stedim Biotech, cat. no. VS2001) at room temperature. The concentration process was stopped at 71 mg/ml due to the high viscosity of the sample. The obtained DLX2323 protein solution was viscous, clear, and no precipitates were observed by visual inspection.
Example 7
The five selected scFvs scFv1-5 were purified and stored in PBS buffer pH 7.2 (Phosphate Buffered Saline 1×, Gibco, Life Technologies™, cat. no. 20012). scFv1 was concentrated using Vivaspin 20 centrifuge concentrators (Sartorius Stedim Biotech, cat. no. VS2001) at room temperature up to 50 mg/mL and analyzed visually and by analytical HPLC (column TOSOH TSKgel G2000 SWXL, cat. no. 08540). The resulting solutions of scFv1 were clear and without any precipitates, and 100% of the protein was monomeric. Thus, the solubility of scFv1 in PBS pH 7.2 is ≥50 mg/mL.
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