WTK1MLL cells were treated as described for the analysis of MLLbcr rearrangements via FACS. Total RNA isolation was done with the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) following the manufacturer´s instructions. Reverse transcription was performed using the QuantiTec Reverse Transcription Kit (Qiagen, Hilden, Germany) following the instructions for 1 µg RNA. For qPCR 3.3 µl cDNA (1:20 diluted) were mixed with 5 µl SensiFAST Probe LO-ROX mix (2x) from the SensiFAST Probe LO-ROX Kit (Bioline, London, UK), 0.5 µl sensor primer and 1.2 µl H2O. The PCR was performed on the qTower (Analytik Jena, Jena, Germany) by using the program 2 min 95°C and 40 cycles of 10 s 95°C and 20 s of 60°C. Controls were ACTB VIC (Thermo Fisher Scientific, Waltham, Massachusetts, USA), HSP90 VIC (Thermo Fisher Scientific, Waltham, Massachusetts, USA),YWHAZ VIC (Thermo Fisher Scientific, Waltham, Massachusetts, USA), ACTB HEX (Bio-Rad Laboratories, Hercules, California, USA) and YWHAZ HEX (Bio-Rad Laboratories, Hercules, California, USA). The genes of interest were analyzed with IFN-gamma Fam, IL-6 Fam, IL-8 Fam and TLR4 Fam (Bio-Rad Laboratories, Hercules, California, USA).
Ywhaz hex
Manufactured by Bio-Rad
Sourced in United States
The YWHAZ HEX is a lab equipment product from Bio-Rad. It is a molecular tool used for the detection and quantification of the YWHAZ gene expression. The core function of this product is to facilitate the measurement of YWHAZ transcript levels in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Qtower, by Analytik Jena (2 mentions)
Sensifast probe lo rox kit, by Meridian Bioscience (2 mentions)
Quantitec reverse transcription kit, by Qiagen (2 mentions)
Rneasy plus mini kit, by Qiagen (2 mentions)
Sensifast probe lo rox mix, by Meridian Bioscience (1 mentions)
Ppia hex, by Bio-Rad (1 mentions)
Rev3l fam, by Bio-Rad (1 mentions)
2 protocols using ywhaz hex
1
Quantitative Analysis of MLL-bcr Rearrangements
2
Quantitative PCR Gene Expression Analysis
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Total RNA was isolated by use of RNeasy Plus Mini Kit (Qiagen) according to the manufacturer′s instructions. Reverse transcription was performed using the QuantiTec Reverse Transcription Kit (Qiagen) following the instructions for 1 μg RNA. Quantitative PCR was performed as described in Eberle et al. (48 (link)) using SensiFAST Probe LO-ROX mix (2×) from the SensiFAST Probe LO-ROX Kit (Bioline, London, UK). The PCR was performed on the qTower (Analytik Jena, Jena, Germany) by using the program 2 min 95°C and 40 cycles of 10 s 95°C and 20 s of 60°C. Controls were PPIA HEX, RPLP0 HEX, YWHAZ HEX (all three BioRad Laboratories, San Diego, USA). The genes of interest were analyzed with REV3L Fam, REV1 Fam, SMARCAL1 Fam, ZRANB3 Fam (BioRad Laboratories, San Diego, USA).
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