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2 protocols using psmd2

1

Antibody Sourcing for Protein Analysis

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Antibodies (Abs) anti-P4D1 ubiquitin, -β2, -PSMD2, -p62 were purchased from Santa Cruz Biotechnology (CA). Abs anti-α6 and -PSMD3 were obtained from Invitrogen. Anti-β-actin Ab was from Abgent. Anti-annexin 5 Ab was from BD Science. Abs anti-β1, -RPN10 and -RPT4 were from Enzo Life Science. Anti-β5, -PSMA2, -LC3B, -ADRM1, -AMBRA1 and -HYOU1 were from Cell Signaling Technology (Danvers, MA, USA). Abs anti-mTOR and -S100A9 were from Sigma (St. Louis, MO, USA). Abs anti-UVRAG, -TOLLIP, -VPS33A, and -VTI1A were from Thermo Fisher Scientific (Waltham, MA, USA). Donkey anti-rabbit TexRed and donkey anti-mouse FITC secondary Abs were from Jackson Immunoresearch. Goat anti-mouse Star 635 was from Abberior and Donkey anti-Rabbit Alexa Fluor 594 was from Invitrogen. Fluorescent secondary Abs IRDye® 800CW and IRDye® 680RD were from LICOR. Peroxidase Goat Anti-Rabbit IgG and Peroxidase Rabbit Anti-Mouse IgG were from Jackson Immunoresearch.
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2

Proteasome Subunit Antibody Analysis

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NF-κB p65, p50, and p105 subunits antibodies and Ik-β and anti-rabbit secondary antibody were obtained from Cell Signaling Technology (MA, USA). The β-actin, anti-ubiquitin, anti-biotin, PSMD2, PSMD3, PSMD11, 20S Proteasome β1, 20S Proteasome α1, polyclonal anti-mouse secondary antibodies, and Protein A/G plus agarose beads were from Santa Cruz Biotechnology (CA, USA). Anti-PSMD1 and anti-TBP antibodies were from Sigma-Aldrich (Dorset, UK).
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