Ma15 evo 18

Manufactured by Zeiss

The MA15/EVO 18 is a scanning electron microscope (SEM) designed and manufactured by Zeiss. It is a versatile instrument capable of high-resolution imaging and analysis of a wide range of samples. The core function of the MA15/EVO 18 is to provide users with detailed, high-quality images and data about the microstructure and composition of their samples.

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Lab products found in correlation

Escalab 250xi, by Thermo Fisher Scientific (1 mentions) Labram hr800, by Horiba (1 mentions)

Spelling variants of this product

EVO 18 (269 citations) EVO MA15 (86 citations)

3 protocols using ma15 evo 18

Scaffold Characterization via SEM and Porosity

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The shape and morphology of prepared scaffolds and the cell–material interaction were studied using scanning electron microscopy (SEM, Carl Zeiss MA15/EVO 18) at an accelerated voltage of 15 keV. The scaffold was coated with gold using a sputter coater (Quorum). The pore size measurements were carried out for the obtained SEM micrographs using Image J software (National Institute of Health, Bethesda, MD, USA). Porosity measurement of the scaffolds was carried out using solvent replacement method. The dried scaffolds were immersed in ethanol overnight and then excess ethanol on the surface was dried before weighing. The porosity of the scaffolds was calculated using the formula

Porosity = (M_{2} - M_{1}) / ρ V \times 100

where, M₁ and M₂ are the mass of the scaffold before and after immersion in ethanol, respectively, ρ is the density of absolute ethanol and V is the volume of the scaffold [24 (link), 25 ].

Martin C.A., Radhakrishnan S., Gómez Ribelles J.L., Trentz O.A., EAK N., Reddy M.S., Rela M, & Kalkura Subbaraya N. (2022). Adipose tissue derived stromal cells in a gelatin-based 3D matrix with exclusive ascorbic acid signalling emerged as a novel neural tissue engineering construct: an innovative prototype for soft tissue. Regenerative Biomaterials, 9, rbac031.

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Comprehensive Characterization of Hydrogel Composites

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Thermogravimetric analysis of hydrogel composites was conducted on a Netzsch TG instrument under nitrogen atmosphere and the heating rate of 10 °C min⁻¹ in the temp range from room temp to 700 °C. XPS was performed using an ESCALAB250Xi photoelectron spectrometer (ThermoFisher Scientific, USA) with X-ray radiation. The crystalline structure of hydrogel was examined by X-ray diffractometer (PAN analytical X’pert PRO) at 30 kV, and 40 mA was used as X-ray source. Raman spectra (LabRAM HR800, Horiba Jobin Yvon) were recorded to investigate the crystalline structure. SEM analysis was performed Carl Zeiss MA15/EVO 18). FTIR spectra of hydrogel materials were recorded by Alfa Bruker FTIR Spectrophotometer.

Zhu L, & Chen L. (2021). Facile design and development of nano-clustery graphene-based macromolecular protein hydrogel loaded with ciprofloxacin to antibacterial improvement for the treatment of burn wound injury. Polymer Bulletin (Berlin, Germany), 79(9), 7953-7968.

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Scaffold Surface Morphology Analysis

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The surface morphology of PG11, PG12 and PG14 scaffolds were studied using SEM (Carl Zeiss MA15/EVO 18) at an accelerated voltage of 15 keV. The scaffold was coated with gold using a sputter coater (Quorum). The SEM micrographs of the scaffolds with cells were captured at 5^th day after seeding 5 × 10⁴ cells onto the surface of the scaffold. These cells were washed with DPBS and the cells were fixed with 4% paraformaldehyde. The paraformaldehyde was washed off with deionized water after 4 hours. This cell laden scaffold was lyophilized as mentioned before (2.2), after pre-freezing. Since porosity is an important feature of the scaffolds which supports the attachment and proliferation of cells, a comparative study was carried out for the obtained SEM micrographs using Image J software (National Institute of Health, Bethesda, MD, USA).

Martin C.A., Radhakrishnan S., Nagarajan S., Muthukoori S., Dueñas J.M., Gómez Ribelles J.L., Lakshmi B.S., E. A. K. N., Gómez-Tejedor J.A., Reddy M.S., Sellathamby S., Rela M, & Subbaraya N.K. (2019). An innovative bioresorbable gelatin based 3D scaffold that maintains the stemness of adipose tissue derived stem cells and the plasticity of differentiated neurons. RSC Advances, 9(25), 14452-14464.

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