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Fvmpe rs microscopy

Manufactured by Olympus
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The FVMPE-RS is a high-performance confocal laser scanning microscope system developed by Olympus. It is designed to provide precise and efficient imaging capabilities for a wide range of applications in life science research. The system features advanced optics, state-of-the-art detectors, and flexible configurations to accommodate various experimental requirements.

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Lab products found in correlation

Lsm 980, by Zeiss (1 mentions) A1r laser scanning confocal microscopy, by Nikon (1 mentions)

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FVMPE-RS

2 protocols using fvmpe rs microscopy

1

Collagen Fiber Analysis via SHG and 2P-FLIM

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Confocal images were conducted by Zeiss LSM 980. To investigate collagen fibers, second-harmonic generation (SHG) and two-photon excited fluorescence imaging were conducted by two-photon Olympus FVMPE-RS microscopy [12 ].
Lin S., Tao C., Yan Q., Gao H., Qin L., Zhong Y., Yao Q., Zhang P., Yang J., Zou X, & Xiao G. (2024). Pip5k1c expression in osteocytes regulates bone remodeling in mice. Journal of Orthopaedic Translation, 45, 36-47.
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2

Immunofluorescence Staining of Paraffin-Embedded Bone Samples

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Immunofluorescence staining with paraffin samples were conducted as described in Refs. [39 ,40 (link)]. Specifically, paraffin bone samples were cut in 5 ​μm thickness, followed by standard re-hydration process, penetrated with 0.25% Triton X-100, blocked with 1% BSA and then incubated with antibodies. Antibodies used in this study are listed in Table 2. Confocal images were conducted by Nikon A1R laser-scanning confocal microscopy with 0.5 ​μm z-stack interval. Second-harmonic generation (SHG) and two-photon excited fluorescence imaging were conducted to investigate collagen fibers with inverted two-photon Olympus FVMPE-RS microscopy.
Qin L., He T., Yang D., Wang Y., Li Z., Yan Q., Zhang P., Chen Z., Lin S., Gao H., Yao Q., Xu Z., Tang B., Yi W, & Xiao G. (2022). Osteocyte β1 integrin loss causes low bone mass and impairs bone mechanotransduction in mice. Journal of Orthopaedic Translation, 34, 60-72.
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