Neoagarotetraose

Manufactured by Biosynth

Sourced in United States, United Kingdom

Neoagarotetraose is a disaccharide compound derived from the marine polysaccharide agarose. It is a structural component of certain types of red algae. Neoagarotetraose can be used as a reference standard or analytical tool in various research and testing applications.

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Lab products found in correlation

Neoagarobiose, by Biosynth (2 mentions) Neoagarohexaose, by Biosynth (2 mentions) Silica gel 60 tlc plate, by Merck Group (2 mentions) D galactose, by Merck Group (1 mentions)

2 protocols using neoagarotetraose

Thin-Layer Chromatography Analysis of rGaa16Bc Hydrolysis

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TLC was used to analyze the hydrolytic patterns of rGaa16Bc. D-galactose (Sigma-Aldrich, Burlington, MA, USA), neoagarobiose (NA2), neoagarotetraose (NA4), and neoagarohexaose (NA6) (all from Carbosynth, Compton, Berkshire, UK) were used as standards. The hydrolytic products were applied to a silica gel 60 TLC plate (Merck, Darmstadt, Germany), which was developed using a solvent system of n-butanol: acetic acid: dH2O (2:1:1 (v/v)). Spots were visualized by spraying orcinol dip reagent (80 mg of orcine monohydrate was dissolved in 160 mL of acetone, to which 8 mL of sulfuric acid was added), followed by heating at 110 °C in a drying oven for 10 min.

Lee Y., Jo E., Lee Y.J., Eom T.Y., Gang Y., Kang Y.H., Marasinghe S.D., Hettiarachchi S.A., Kang D.H, & Oh C. (2021). A Novel Agarase, Gaa16B, Isolated from the Marine Bacterium Gilvimarinus agarilyticus JEA5, and the Moisturizing Effect of Its Partial Hydrolysis Products. Marine Drugs, 20(1), 2.

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Thin-layer Chromatographic Analysis of Agarose Hydrolysis

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The hydrolytic product of agarose from the recombinant enzyme rGaa16A was identified using thin-layer chromatography (TLC). Enzymatic hydrolysis of agarose was carried out at 45°C for 1 h in distilled water containing 10 μl of rGaa16A and 90 μl of 0.5% agarose. The mixture was applied to a silica gel 60 TLC plate (Merck, Germany) and developed with n-butanol:acetic acid:dH 2 O (2:1:1 (v/v)). Spots were visualized by spraying with an orcinol dip reagent (80 mg of orcine monohydrate dissolved in 160 ml of acetone; 8 ml of sulfuric acid then added), followed by heating at 100°C for 10 min. D-(+)-Galactose (Riedel de Haen, Germany), neoagarobiose (Carbosynth, UK), neoagarotetraose (Carbosynth, UK), and neoagarohexaose (Carbosynth, UK) were used as standards.

Lee Y., Jo E., Lee Y.J., Hettiarachchi S.A., Park G.H., Lee S.J., Heo S.J., Kang D.H, & Oh C. (2018). A Novel Glycosyl Hydrolase Family 16 β-Agarase from the Agar-Utilizing Marine Bacterium Gilvimarinus agarilyticus JEA5: the First Molecular and Biochemical Characterization of Agarase in Genus Gilvimarinus. Journal of microbiology and biotechnology, 28(5).

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